Western blot abreview for Anti-Caveolin 3 antibody - Caveolae Marker

Good
Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - whole (Heart)
Loading amount
50 µg
Specification
Heart
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Other product details

Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 5°C · Diluent: 5%milk in PBS-T20

Secondary antibody

Name
Non-Abcam antibody was used: Swine anti-Rabbit
Host species: Pig
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000

Detection

Detection method
ECL
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: approximately 20KDa kDa

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Submitted Dec 28 2011

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