Western blot abreview for Anti-Caveolin-3 antibody - Caveolae Marker

Excellent
Abreviews
Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Reduced Denaturing (12.5%)
Sample
Sheep Tissue lysate - whole (Cardiac)
Specification
Cardiac
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Other product details

Incubation time
1 hour(s) and 30 minute(s) · Temperature: 22°C · Diluent: 1% milk TBST
Dilution
1/3000

Secondary antibody

Name
Non-Abcam antibody was used: Donkey Anti-Rabbit IgG (H+L)
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000

Detection

Detection method
ECL
Bands
Specific: 15-20 kDa
Exposure
30 second(s)
Positive control
5 x samples of sheep cardiac tissue, lysed in RIPA with protease inhibitors

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Submitted Nov 20 2013

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