Anti-CBARA1 antibody (ab115025)


  • Product name
  • Description
    Goat polyclonal to CBARA1
  • Host species
  • Specificity
    ab115025 is expected to recognize reported isoforms 1 and 2 (NP_006068.2; NP_001182447.1).
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog, Pig
  • Immunogen

    Synthetic peptide:


    , corresponding to internal sequence amino acids 113-126 of Human CBARA1 (NP_006068.2; NP_001182447.1). (Peptide available as ab126461.)

  • Positive control
    • K562 lysate.



Our Abpromise guarantee covers the use of ab115025 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).Can be blocked with Human CBARA1 peptide (ab126461). An additional band of unknown identity was also consistently observed at 37kDa. This band was successfully blocked by incubation with the immunizing peptide.


  • Function
    Key regulator of mitochondrial calcium uptake required for calcium entry into mitochondrion. May act as a calcium sensor via its EF-hand domains, gating the activity of a calcium channel partner. Induces T-helper 1-mediated autoreactivity, which is accompanied by the release of IFNG.
  • Tissue specificity
    Expressed in epithelial cell line. Strongly expressed in epidermal keratinocytes and dermal endothelial cells.
  • Sequence similarities
    Belongs to the MICU1 family.
    Contains 2 EF-hand domains.
  • Domain
    The EF-hand domains are required for mitochondrial calcium homeostasis function.
  • Cellular localization
    Mitochondrion inner membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Allergen Hom s 4 antibody
    • ara CALC antibody
    • atopy related autoantigen antibody
    • atopy related autoantigen CALC antibody
    • Atopy-related autoantigen CALC antibody
    • CALC antibody
    • calcium binding atopy related autoantigen 1 antibody
    • Calcium uptake protein 1 antibody
    • calcium uptake protein 1, mitochondrial antibody
    • Calcium-binding atopy-related autoantigen 1 antibody
    • CBARA1 antibody
    • EFHA3 antibody
    • FLJ12684 antibody
    • MICU1 antibody
    • MICU1_HUMAN antibody
    • mitochondrial antibody
    • mitochondrial calcium uptake 1 antibody
    • MPXPS antibody
    see all


  • Anti-CBARA1 antibody (ab115025) at 0.5 µg/ml + K562 lysate (in RIPA buffer) at 35 µg

    Developed using the ECL technique.

    Predicted band size: 55 kDa

    Primary incubation: 1 hour


ab115025 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Thank you for contacting Abcam yesterday.

I have talked to the lab and we will be able to get the blocking peptide for ab115025 added to our catalogue. The product should be added in a day or so, therefore you should be able to purchase it by...

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Western blot
Loading amount
50 µg
Gel Running Conditions
Non-reduced Denaturing (4-12% BIS-TRIS)
Human Cell lysate - whole cell (fibroblasts)
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Abcam user community

Verified customer

Submitted Feb 20 2014

Abcam has not validated the combination of species/application used in this Abreview.
Western blot
Mouse Cell lysate - whole cell (HL-1)
Loading amount
65 µg
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Jan 10 2013

We apologize for typos error. The date should be read as January 30th 2013.

Best of luck with your research.


Expiration date: 30/01/2012

I am very pleased to hear you would like to accept our offer and testab115025 in WB on mouse samples. This code will give you: 1 free PRIMARY ANTIBODY/PROTEIN OR VALUE OFF O...

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Thank you for your reply.

I am sorry that these antibodies did not perform as stated on the datasheet. I have asked our accounting department to issue a credit note for you, which can be redeemed against the invoice of a future order by passin...

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Thank you for this information.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement of ab 135358.

To check the sta...

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Thank you for your reply.

The immunogen sequence used to generate this antibody (ab102830) is excluded from this isoform, so this is not a possibility. Ab115025 would recognize this isoform, but itis not present on your blot.

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Thank you for your reply.

Yes, this antibody is detecting Isoform 4 (MW 32kDa), as opposed to the other two which detected isoform 1 in the samples. You may still detect a 55kDa band in your samples if that is the isoform present.
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Thank you for contacting Abcam regarding these CBARA1 antibodies.

Based on the information and images you provided, I agree that these antibodies are not performing as we would expect. I am happy to offer replacements or refunds for both ...

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