Key features and details
- Rabbit polyclonal to CBF
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-CBF antibody
See all CBF primary antibodies
DescriptionRabbit polyclonal to CBF
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
- HeLa and 293T whole cell lysates.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 99% Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab176579 was affinity purified using an epitope specific to CBF immobilized on solid support.
Our Abpromise guarantee covers the use of ab176579 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 121 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionStimulates transcription from the HSP70 promoter.
Sequence similaritiesBelongs to the CBF/MAK21 family.
- Information by UniProt
- CBF antibody
- CBF2 antibody
- CCAAT binding factor antibody
All lanes : Anti-CBF antibody (ab176579) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysates at 50 µg
Lane 2 : HeLa whole cell lysates at 15 µg
Lane 3 : HeLa whole cell lysates at 5 µg
Lane 4 : 293T whole cell lysates at 50 µg
Developed using the ECL technique.
Predicted band size: 121 kDa
Exposure time: 30 seconds
Detection of CBF in Immunoprecipitates of HeLa whole cell lysates (1 mg for IP, 20% of IP loaded) using ab176579 at 6 µg/mg lysate for IP (Lane 1). For WB detection ab176579 was used at 1 µg/ml. Lane 2 represents control IgG IP.
Detection: Chemiluminescence with an exposure time of 10 seconds.
ab176579 has been referenced in 1 publication.
- Barbieri I et al. Promoter-bound METTL3 maintains myeloid leukaemia by m6A-dependent translation control. Nature 552:126-131 (2017). PubMed: 29186125