Anti-CBFb antibody (ab125191)
Key features and details
- Rabbit polyclonal to CBFb
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-CBFb antibody
See all CBFb primary antibodies -
Description
Rabbit polyclonal to CBFb -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide, corresponding to a region within amino acids 166-187 of Human CBFb (NP_074036.1).
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Positive control
- WB: HeLa, HEK-293T, TCMK, NIH/3T3 and Jurkat whole cell lysate. IP: Jurkat whole cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab125191 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 22 kDa.
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IP |
Use at 2-10 µg/mg of lysate.
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Notes |
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WB
1/2000 - 1/10000. Predicted molecular weight: 22 kDa. |
IP
Use at 2-10 µg/mg of lysate. |
Target
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Function
CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL3 and GM-CSF promoters. CBFB enhances DNA binding by RUNX1. -
Involvement in disease
Note=A chromosomal aberration involving CBFB is associated with acute myeloid leukemia of M4EO subtype. Pericentric inversion inv(16)(p13;q22). The inversion produces a fusion protein that consists of the 165 N-terminal residues of CBF-beta (PEPB2) with the tail region of MYH11. -
Sequence similarities
Belongs to the CBF-beta family. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 865 Human
- Entrez Gene: 12400 Mouse
- Omim: 121360 Human
- SwissProt: Q13951 Human
- SwissProt: Q08024 Mouse
- Unigene: 460988 Human
- Unigene: 2018 Mouse
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Alternative names
- CBF b antibody
- CBF beta antibody
- CBF-beta antibody
see all
Images
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All lanes : Anti-CBFb antibody (ab125191) at 0.1 µg/ml
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : TCMK (Mouse kidney epithelial cell line) whole cell lysate
Lane 5 : NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 22 kDa
Exposure time: 30 secondsCells prepared using NETN lysis buffer.
4-12% SDS-PAGE.
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Detection of human CBFb by western blot of immunoprecipitates.
Lane 1: Sample.
Lane 2: Control IgG.
Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat (Human T cell leukemia cell line from peripheral blood) cells prepared using NETN lysis buffer.
Antibodies: ab125191 used for IP at 3 μg per reaction.For blotting immunoprecipitated CBFb ab125191 was used at 1 μg/ml.
Detection: Chemiluminescence with an exposure time of 30 seconds.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab125191 has been referenced in 5 publications.
- Simeoni F et al. Enhancer recruitment of transcription repressors RUNX1 and TLE3 by mis-expressed FOXC1 blocks differentiation in acute myeloid leukemia. Cell Rep 36:109725 (2021). PubMed: 34551306
- Malik N et al. The transcription factor CBFB suppresses breast cancer through orchestrating translation and transcription. Nat Commun 10:2071 (2019). PubMed: 31061501
- Shin MH et al. A RUNX2-Mediated Epigenetic Regulation of the Survival of p53 Defective Cancer Cells. PLoS Genet 12:e1005884 (2016). PubMed: 26925584
- Huang P et al. Direct reprogramming of human fibroblasts to functional and expandable hepatocytes. Cell Stem Cell 14:370-84 (2014). PubMed: 24582927
- Lopez-Camacho C et al. Core binding factor ß (CBFß) is retained in the midbody during cytokinesis. J Cell Physiol 229:1466-74 (2014). PubMed: 24648201