Product nameAnti-CBFb antibody
See all CBFb primary antibodies
DescriptionRabbit polyclonal to CBFb
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rabbit, Horse, Dog, Chimpanzee, Rhesus monkey
Synthetic peptide, corresponding to a region within amino acids 166-187 of Human CBFb (NP_074036.1).
- Jurkat whole cell lysate HeLa whole cell lysate 293T whole cell lysate
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab125191 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 22 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionCBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL3 and GM-CSF promoters. CBFB enhances DNA binding by RUNX1.
Involvement in diseaseNote=A chromosomal aberration involving CBFB is associated with acute myeloid leukemia of M4EO subtype. Pericentric inversion inv(16)(p13;q22). The inversion produces a fusion protein that consists of the 165 N-terminal residues of CBF-beta (PEPB2) with the tail region of MYH11.
Sequence similaritiesBelongs to the CBF-beta family.
- Information by UniProt
- CBF b antibody
- CBF beta antibody
- CBF-beta antibody
All lanes : Anti-CBFb antibody (ab125191) at 0.1 µg/ml
Lane 1 : Jurkat whole cell lysate at 50 µg
Lane 2 : Jurkat whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 50 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 22 kDa
Exposure time: 3 minutes
Immunoprecipitation : 1 mg of Jurkat cell lysate immunoprecipitated with ab125191 at 6 µg/mg lysate; 20% of immunoprecipitate loaded in lane
Western Blot: CBFb antibody (ab125191) at 1 µg/ml
developed using the ECL technique
Exposure time : 30 seconds
ab125191 has not yet been referenced specifically in any publications.