Key features and details
- Rabbit polyclonal to CBFb
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-CBFb antibody
See all CBFb primary antibodies
DescriptionRabbit polyclonal to CBFb
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide, corresponding to a region within amino acids 166-187 of Human CBFb (NP_074036.1).
- WB: HeLa, HEK-293T, TCMK, NIH/3T3 and Jurkat whole cell lysate. IP: Jurkat whole cell lysate.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab125191 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 22 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionCBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL3 and GM-CSF promoters. CBFB enhances DNA binding by RUNX1.
Involvement in diseaseNote=A chromosomal aberration involving CBFB is associated with acute myeloid leukemia of M4EO subtype. Pericentric inversion inv(16)(p13;q22). The inversion produces a fusion protein that consists of the 165 N-terminal residues of CBF-beta (PEPB2) with the tail region of MYH11.
Sequence similaritiesBelongs to the CBF-beta family.
- Information by UniProt
- CBF b antibody
- CBF beta antibody
- CBF-beta antibody
All lanes : Anti-CBFb antibody (ab125191) at 0.1 µg/ml
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : TCMK (Mouse kidney epithelial cell line) whole cell lysate
Lane 5 : NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 22 kDa
Exposure time: 30 seconds
Cells prepared using NETN lysis buffer.
Detection of human CBFb by western blot of immunoprecipitates.
Lane 1: Sample.
Lane 2: Control IgG.
Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat (Human T cell leukemia cell line from peripheral blood) cells prepared using NETN lysis buffer.
Antibodies: ab125191 used for IP at 3 μg per reaction.For blotting immunoprecipitated CBFb ab125191 was used at 1 μg/ml.
Detection: Chemiluminescence with an exposure time of 30 seconds.
ab125191 has been referenced in 4 publications.
- Malik N et al. The transcription factor CBFB suppresses breast cancer through orchestrating translation and transcription. Nat Commun 10:2071 (2019). PubMed: 31061501
- Shin MH et al. A RUNX2-Mediated Epigenetic Regulation of the Survival of p53 Defective Cancer Cells. PLoS Genet 12:e1005884 (2016). PubMed: 26925584
- Huang P et al. Direct reprogramming of human fibroblasts to functional and expandable hepatocytes. Cell Stem Cell 14:370-84 (2014). PubMed: 24582927
- Lopez-Camacho C et al. Core binding factor ß (CBFß) is retained in the midbody during cytokinesis. J Cell Physiol 229:1466-74 (2014). PubMed: 24648201