Recombinant
RabMAb

Recombinant Anti-CBL + CBLB antibody [YE315] - BSA and Azide free (ab223584)

Overview

  • Product name

    Anti-CBL + CBLB antibody [YE315] - BSA and Azide free
  • Description

    Rabbit monoclonal [YE315] to CBL + CBLB - BSA and Azide free
  • Host species

    Rabbit
  • Specificity

    The immunogen sequence is 100% identical for both CBL and CBLB, therefore there is likely to be cross-reactivity.

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chicken
  • Immunogen

    Synthetic peptide within Human CBL aa 450-550. The exact sequence is proprietary.
    Database link: P22681

  • Positive control

    • WB: HuT-78, HeLa and Jurkat cell lysate. IHC-P: Human uterus adenocarcinoma and colon tissues. ICC/IF: Jurkat cells.
  • General notes

    ab223584 is a PBS-only buffer format of ab32425. Please refer to ab32425 for recommended dilutions, protocols, and image data.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Applications

Our Abpromise guarantee covers the use of ab223584 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 120 kDa (predicted molecular weight: 99 kDa).
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

ICC/IF Use at an assay dependent concentration.

Target

  • Relevance

    CBL: Adapter protein that functions as a negative regulator of many signaling pathways that are triggered by activation of cell surface receptors. Acts as an E3 ubiquitin-protein ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and then transfers it to substrates promoting their degradation by the proteasome. Recognizes activated receptor tyrosine kinases, including KIT, FLT1, FGFR1, FGFR2, PDGFRA, PDGFRB, EGFR, CSF1R, EPHA8 and KDR and terminates signaling. Recognizes membrane-bound HCK, SRC and other kinases of the SRC family and mediates their ubiquitination and degradation. Participates in signal transduction in hematopoietic cells. Plays an important role in the regulation of osteoblast differentiation and apoptosis. Essential for osteoclastic bone resorption. The 'Tyr-731' phosphorylated form induces the activation and recruitment of phosphatidylinositol 3-kinase to the cell membrane in a signaling pathway that is critical for osteoclast function. May be functionally coupled with the E2 ubiquitin-protein ligase UB2D3. Pathway:protein ubiquitination. This protein is involved in the pathway protein ubiquitination, which is part of Protein modification. CBLB: E3 ubiquitin-protein ligase which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and transfers it to substrates, generally promoting their degradation by the proteasome. Negatively regulates TCR (T-cell receptor), BCR (B-cell receptor) and FCER1 (high affinity immunoglobulin epsilon receptor) signal transduction pathways. In naive T-cells, inhibits VAV1 activation upon TCR engagement and imposes a requirement for CD28 costimulation for proliferation and IL-2 production. Also acts by promoting PIK3R1/p85 ubiquitination, which impairs its recruitment to the TCR and subsequent activation. In activated T-cells, inhibits PLCG1 activation and calcium mobilization upon restimulation and promotes anergy. In B-cells, acts by ubiquitinating SYK and promoting its proteasomal degradation. Slightly promotes SRC ubiquitination. May be involved in EGFR ubiquitination and internalization. May be functionally coupled with the E2 ubiquitin-protein ligase UB2D3. Pathway: protein ubiquitination. This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
  • Cellular localization

    CBL: Cytoplasm. CBLB: Cytoplasmic and Nuclear
  • Database links

References

ab223584 has not yet been referenced specifically in any publications.

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