Product nameAnti-Cbl-c antibody
See all Cbl-c primary antibodies
DescriptionRabbit polyclonal to Cbl-c
SpecificityNo reactivity is expected with Cbl-a or Cbl-b.
Tested applicationsSuitable for: ELISA, WB, IP, ICC/IF, IHC-Pmore details
Predicted to work with: Mouse, Rat, Human, Chimpanzee
- Hek 293T cell lysate.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThe product was affinity purified from monospecific antiserum by immunoaffinity purification.
Our Abpromise guarantee covers the use of ab34750 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||1/10000 - 1/50000.|
|WB||1/500 - 1/3000. Predicted molecular weight: 52 kDa.|
|IP||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration.|
RelevanceCblc belongs to a family of ubiquitin protein ligases (E3s) that negatively regulate signaling by targeting activated tyrosine kinases for degradation. Cblc is the most recently cloned member of the Cbl proteins and is expressed only in epithelial cells (the other Cbl proteins are ubiquitous). Cblc, like the other mammalian Cbl proteins, can ubiquitinate the activated EGFR and target it for degradation. Cblc knock out mice show no obvious phenotype. Thus, the physiological role of Cblc is not known.
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All lanes : Anti-Cbl-c antibody (ab34750) at 1/500 dilution
Lanes 1 & 3 : Hek 293T cell lysate transfected with empty vector
Lanes 2 & 4 : Hek 293T cell lysate transfected with Cbl-c.
Predicted band size: 52 kDa
Observed band size: 52 kDa
The right panel shows western blotting after first immunoprecipitating with Rabbit anti- Cbl-c followed by immunoblotting.
Image courtesy of Human Protein Atlas
ab34750 (1/500) showing strong nuclear and cytoplasmic stainning of cells of the kidney tubuli. Paraffin embedded human kidney tissue was incubated with ab123 (1/500 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.
ab34750 has not yet been referenced specifically in any publications.