Western blot abreview for Anti-CBL (phospho S669) antibody [EPR2226(2)]

Average
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293)
Loading amount
30 µg
Specification
HEK293
Treatment
Activator
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 20°C

Other product details

Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS + BSA

Secondary antibody

Name
Non-Abcam antibody was used: goat anti rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 680
Dilution
1/10000

Detection

Detection method
LiCor
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 110 kDa Non-specific: 65, 30, 20 approx kDa
Positive control
lane 2: Treated with activator
Negative control
lane 1: control

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Submitted May 10 2013

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