Recombinant
RabMAb

Recombinant Anti-CBL (phospho S669) antibody [EPR2226(2)] - BSA and Azide free (ab247648)

Overview

  • Product name

    Anti-CBL (phospho S669) antibody [EPR2226(2)] - BSA and Azide free
    See all CBL primary antibodies
  • Description

    Rabbit monoclonal [EPR2226(2)] to CBL (phospho S669) - BSA and Azide free
  • Host species

    Rabbit
  • Specificity

    This antibody only detects Cbl phosphorylated at Serine 669. 

  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human CBL (phospho S669). The exact sequence is proprietary.

  • General notes

    Ab247648 is the carrier-free version of ab108364. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab247648 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Applications

Our Abpromise guarantee covers the use of ab247648 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 100 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • Target

    • Function

      Participates in signal transduction in hematopoietic cells. Adapter protein that functions as a negative regulator of many signaling pathways that start from receptors at the cell surface. Acts as an E3 ubiquitin-protein ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and then transfers it to substrates promoting their degradation by the proteasome. Recognizes activated receptor tyrosine kinases, including PDGFA, EGF and CSF1, and terminates signaling.
    • Pathway

      Protein modification; protein ubiquitination.
    • Involvement in disease

      Defects in CBL are the cause of Noonan syndrome-like disorder (NSL) [MIM:613563]. NSL is a syndrome characterized by a phenotype reminiscent of Noonan syndrome. Clinical features are highly variable, including facial dysmorphism, short neck, developmental delay, hyperextensible joints and thorax abnormalities with widely spaced nipples. The facial features consist of triangular face with hypertelorism, large low-set ears, ptosis, and flat nasal bridge. Some patients manifest cardiac defects.
    • Sequence similarities

      Contains 1 Cbl-PTB (Cbl-type phosphotyrosine-binding) domain.
      Contains 1 RING-type zinc finger.
      Contains 1 UBA domain.
    • Domain

      The RING-type zinc finger domain mediates binding to an E2 ubiquitin-conjugating enzyme.
      The N-terminus is composed of the phosphotyrosine binding (PTB) domain, a short linker region and the RING-type zinc finger. The PTB domain, which is also called TKB (tyrosine kinase binding) domain, is composed of three different subdomains: a four-helix bundle (4H), a calcium-binding EF hand and a divergent SH2 domain.
    • Post-translational
      modifications

      Phosphorylated on tyrosine residues by EGFR, SYK, FYN and ZAP70 (By similarity). Phosphorylated on tyrosine residues by INSR.
    • Cellular localization

      Cytoplasm.
    • Information by UniProt
    • Database links

    • Alternative names

      • 4732447J05Rik antibody
      • C CBL antibody
      • Cas Br M (murine) ecotropic retroviral transforming sequence antibody
      • Casitas B lineage lymphoma proto oncogene antibody
      • Casitas B-lineage lymphoma proto-oncogene antibody
      • CBL 2 antibody
      • cbl antibody
      • CBL_HUMAN antibody
      • CBL2 antibody
      • E3 ubiquitin protein ligase CBL antibody
      • E3 ubiquitin-protein ligase CBL antibody
      • Oncogene CBL2 antibody
      • Proto oncogene c CBL antibody
      • Proto-oncogene c-CBL antibody
      • RGD1561386 antibody
      • RING finger protein 55 antibody
      • RNF55 antibody
      • Signal transduction protein CBL antibody
      see all

    References

    ab247648 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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