Anti-CBL (phospho Y700) antibody [EP2225Y] - BSA and Azide free (ab284732)
![Western blot - Anti-CBL (phospho Y700) antibody [EP2225Y] - BSA and Azide free (ab284732)](https://www.abcam.com/ps/products/284/ab284732/Images/ab284732-476399-anti-cbl-Jurkat-cell-lysate-untreated-phospho-y700-antibody-ep2225y-western-blot.gif "Western blot - Anti-CBL (phospho Y700) antibody [EP2225Y] - BSA and Azide free (ab284732)")
Key features and details
- Rabbit monoclonal [EP2225Y] to CBL (phospho Y700) - BSA and Azide free
- Suitable for: WB, Flow Cyt (Intra)
- Reacts with: Human
- Isotype: IgG
Related conjugates and formulations
Overview
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Product name
Anti-CBL (phospho Y700) antibody [EP2225Y] - BSA and Azide free
See all CBL primary antibodies -
Description
Rabbit monoclonal [EP2225Y] to CBL (phospho Y700) - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, Flow Cyt (Intra)more details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Jurkat cell lysate, treated with pervanadate Flow Cyt: Permeabilized Jurkat cells, treated with pervanadate.
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General notes
ab284732 is the carrier-free version of ab76002
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.2
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP2225Y -
Isotype
IgG -
Research areas
- Cell Biology
- Proteolysis / Ubiquitin
- Proteasome / Ubiquitin
- Ubiquitin E3 Enzymes
- RING Finger E3 Ligase
- Signal Transduction
- Signaling Pathway
- Nuclear Signaling
- Nuclear Hormone Receptors
- Co-activators/co-repressors
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab284732 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use at an assay dependent concentration. Predicted molecular weight: 100 kDa.
|
|
| Flow Cyt (Intra) |
Use at an assay dependent concentration.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
| Notes |
|---|
|
WB
Use at an assay dependent concentration. Predicted molecular weight: 100 kDa. |
|
Flow Cyt (Intra)
Use at an assay dependent concentration. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
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Function
Participates in signal transduction in hematopoietic cells. Adapter protein that functions as a negative regulator of many signaling pathways that start from receptors at the cell surface. Acts as an E3 ubiquitin-protein ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and then transfers it to substrates promoting their degradation by the proteasome. Recognizes activated receptor tyrosine kinases, including PDGFA, EGF and CSF1, and terminates signaling. -
Pathway
Protein modification; protein ubiquitination. -
Involvement in disease
Defects in CBL are the cause of Noonan syndrome-like disorder (NSL) [MIM:613563]. NSL is a syndrome characterized by a phenotype reminiscent of Noonan syndrome. Clinical features are highly variable, including facial dysmorphism, short neck, developmental delay, hyperextensible joints and thorax abnormalities with widely spaced nipples. The facial features consist of triangular face with hypertelorism, large low-set ears, ptosis, and flat nasal bridge. Some patients manifest cardiac defects. -
Sequence similarities
Contains 1 Cbl-PTB (Cbl-type phosphotyrosine-binding) domain.
Contains 1 RING-type zinc finger.
Contains 1 UBA domain. -
Domain
The RING-type zinc finger domain mediates binding to an E2 ubiquitin-conjugating enzyme.
The N-terminus is composed of the phosphotyrosine binding (PTB) domain, a short linker region and the RING-type zinc finger. The PTB domain, which is also called TKB (tyrosine kinase binding) domain, is composed of three different subdomains: a four-helix bundle (4H), a calcium-binding EF hand and a divergent SH2 domain. -
Post-translational
modificationsPhosphorylated on tyrosine residues by EGFR, SYK, FYN and ZAP70 (By similarity). Phosphorylated on tyrosine residues by INSR. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 867 Human
- Omim: 165360 Human
- SwissProt: P22681 Human
- Unigene: 504096 Human
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Alternative names
- 4732447J05Rik antibody
- C CBL antibody
- Cas Br M (murine) ecotropic retroviral transforming sequence antibody
see all
Images
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Western blot - Anti-CBL (phospho Y700) antibody [EP2225Y] - BSA and Azide free (ab284732)All lanes : Anti-CBL (phospho Y700) antibody [EP2225Y] (ab76002) at 1/10000 dilution
Lane 1 : Jurkat cell lysate, untreated
Lane 2 : Jurkat cell lysate, treated with pervanadate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 100 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?This data was developed using ab76002, the same antibody clone in a different buffer formulation.
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![Flow Cytometry (Intracellular) - Anti-CBL (phospho Y700) antibody [EP2225Y] - BSA and Azide free (ab284732)](https://www.abcam.com/ps/products/284/ab284732/Images/ab284732-2-anti-cbl-phospho-AQ73y700-antibody-ep2225y-flow-cytometry.gif)
Flow Cytometry (Intracellular) - Anti-CBL (phospho Y700) antibody [EP2225Y] - BSA and Azide free (ab284732)This data was developed using ab76002, the same antibody clone in a different buffer formulation.Intracellular flow cytometric analysis of ab76002 at 1/50 dilution staining permeabilized Jurkat cells, untreated (green) or pervanadate-treated (red) and pervanadate-treated Jurkat cells preincubated with immunising phospho-peptide (blue) or non-phospho peptide (orange).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab284732 has not yet been referenced specifically in any publications.
