RecombinantRabMAb

Recombinant Anti-CBL (phospho Y774) antibody [E160] - BSA and Azide free (ab247260)

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Western blot - Anti-CBL (phospho Y774) antibody [E160] - BSA and Azide free (ab247260)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CBL (phospho Y774) antibody [E160] - BSA and Azide free (ab247260)
  • Flow Cytometry - Anti-CBL (phospho Y774) antibody [E160] - BSA and Azide free (ab247260)
  • Anti-CBL (phospho Y774) antibody [E160] - BSA and Azide free (ab247260)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [E160] to CBL (phospho Y774) - BSA and Azide free
  • Suitable for: IHC-P, ICC/IF, WB, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-CBL (phospho Y774) antibody [E160] - BSA and Azide free
    See all CBL primary antibodies

  • Description

    Rabbit monoclonal [E160] to CBL (phospho Y774) - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    The antibody detects Cbl phosphorylated on Tyrosine 774. 

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab247260 is the carrier-free version of ab32446 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab247260 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab247260 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
Flow Cyt
Human
IHC-P
Human
WB
Human
Application Abreviews Notes
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 120 kDa (predicted molecular weight: 99 kDa).
Flow Cyt
Use at an assay dependent concentration.
Notes
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 120 kDa (predicted molecular weight: 99 kDa).
Flow Cyt
Use at an assay dependent concentration.

Target

  • Function

    Participates in signal transduction in hematopoietic cells. Adapter protein that functions as a negative regulator of many signaling pathways that start from receptors at the cell surface. Acts as an E3 ubiquitin-protein ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and then transfers it to substrates promoting their degradation by the proteasome. Recognizes activated receptor tyrosine kinases, including PDGFA, EGF and CSF1, and terminates signaling.

  • Pathway

    Protein modification; protein ubiquitination.

  • Involvement in disease

    Defects in CBL are the cause of Noonan syndrome-like disorder (NSL) [MIM:613563]. NSL is a syndrome characterized by a phenotype reminiscent of Noonan syndrome. Clinical features are highly variable, including facial dysmorphism, short neck, developmental delay, hyperextensible joints and thorax abnormalities with widely spaced nipples. The facial features consist of triangular face with hypertelorism, large low-set ears, ptosis, and flat nasal bridge. Some patients manifest cardiac defects.

  • Sequence similarities

    Contains 1 Cbl-PTB (Cbl-type phosphotyrosine-binding) domain.
    Contains 1 RING-type zinc finger.
    Contains 1 UBA domain.

  • Domain

    The RING-type zinc finger domain mediates binding to an E2 ubiquitin-conjugating enzyme.
    The N-terminus is composed of the phosphotyrosine binding (PTB) domain, a short linker region and the RING-type zinc finger. The PTB domain, which is also called TKB (tyrosine kinase binding) domain, is composed of three different subdomains: a four-helix bundle (4H), a calcium-binding EF hand and a divergent SH2 domain.

  • Post-translational
    modifications

    Phosphorylated on tyrosine residues by EGFR, SYK, FYN and ZAP70 (By similarity). Phosphorylated on tyrosine residues by INSR.

  • Cellular localization

    Cytoplasm.
  • Information by UniProt

  • Database links

    • Alternative names

      • 4732447J05Rik antibody
      • C CBL antibody
      • Cas Br M (murine) ecotropic retroviral transforming sequence antibody
      • Casitas B lineage lymphoma proto oncogene antibody
      • Casitas B-lineage lymphoma proto-oncogene antibody
      • CBL 2 antibody
      • cbl antibody
      • CBL_HUMAN antibody
      • CBL2 antibody
      • E3 ubiquitin protein ligase CBL antibody
      • E3 ubiquitin-protein ligase CBL antibody
      • Oncogene CBL2 antibody
      • Proto oncogene c CBL antibody
      • Proto-oncogene c-CBL antibody
      • RGD1561386 antibody
      • RING finger protein 55 antibody
      • RNF55 antibody
      • Signal transduction protein CBL antibody
      see all

    Images

    • Western blot - Anti-CBL (phospho Y774) antibody [E160] - BSA and Azide free (ab247260)
      Western blot - Anti-CBL (phospho Y774) antibody [E160] - BSA and Azide free (ab247260)
      All lanes : Anti-CBL (phospho Y774) antibody [E160] (ab32446) at 1/1000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : Jurkat cell lysate + Pervanadate

      Predicted band size: 99 kDa
      Observed band size: 120 kDa why is the actual band size different from the predicted?



      This data was developed using ab32446, the same antibody clone in a different buffer formulation.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CBL (phospho Y774) antibody [E160] - BSA and Azide free (ab247260)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CBL (phospho Y774) antibody [E160] - BSA and Azide free (ab247260)
      This data was developed using ab32446, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of human cervical carcinoma using ab32446 at 1/25 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
    • Flow Cytometry - Anti-CBL (phospho Y774) antibody [E160] - BSA and Azide free (ab247260)
      Flow Cytometry - Anti-CBL (phospho Y774) antibody [E160] - BSA and Azide free (ab247260)
      This data was developed using ab32446, the same antibody clone in a different buffer formulation.Flow cytometric analysis of permeabilized Jurkat cells, untreated (green) or Pervanadate-treated (red), using ab32446 at 1/20 dilution.
    • Anti-CBL (phospho Y774) antibody [E160] - BSA and Azide free (ab247260)
      Anti-CBL (phospho Y774) antibody [E160] - BSA and Azide free (ab247260)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab247260? Please let us know so that we can cite the reference in this datasheet.

    ab247260 has not yet been referenced specifically in any publications.

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