• Product name

    Anti-CBLB antibody [246C5a]
    See all CBLB primary antibodies
  • Description

    Mouse monoclonal [246C5a] to CBLB
  • Host species

  • Tested applications

    Suitable for: IHC-P, Dot blot, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant C terminal fragment of Human CBLB.



Our Abpromise guarantee covers the use of ab54362 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Dot blot Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 109 kDa.


  • Relevance

    Function: E3 ubiquitin-protein ligase which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and transfers it to substrates, generally promoting their degradation by the proteasome. Negatively regulates TCR (T-cell receptor), BCR (B-cell receptor) and FCER1 (high affinity immunoglobulin epsilon receptor) signal transduction pathways. In naive T-cells, inhibits VAV1 activation upon TCR engagement and imposes a requirement for CD28 costimulation for proliferation and IL-2 production. Also acts by promoting PIK3R1/p85 ubiquitination, which impairs its recruitment to the TCR and subsequent activation. In activated T-cells, inhibits PLCG1 activation and calcium mobilization upon restimulation and promotes anergy. In B-cells, acts by ubiquitinating SYK and promoting its proteasomal degradation. Slightly promotes SRC ubiquitination. May be involved in EGFR ubiquitination and internalization. May be functionally coupled with the E2 ubiquitin-protein ligase UB2D3. Tissue specificity: Expressed in placenta, heart, lung, kidney, spleen, ovary and testis, as well as fetal brain and liver and hematopoietic cell lines, but not in adult brain, liver, pancreas, salivary gland, or skeletal muscle. Present in lymphocytes (at protein level). Pathway: Protein modification; protein ubiquitination. Similarity: Contains 1 Cbl-PTB (Cbl-type phosphotyrosine-binding) domain. Contains 1 RING-type zinc finger. Contains 1 UBA domain. Domain: The N-terminus is composed of the phosphotyrosine binding (PTB) domain, a short linker region and the RING-type zinc finger. The PTB domain, which is also called TKB (tyrosine kinase binding) domain, is composed of three different subdomains: a four-helix bundle (4H), a calcium-binding EF hand and a divergent SH2 domain. The RING-type zinc finger domain mediates binding to an E2 ubiquitin-conjugating enzyme. The UBA domain interacts with poly-ubiquitinated proteins. PTM: Phosphorylated on tyrosine and serine residues upon TCR or BCR activation, and upon various types of cell stimulation. Auto-ubiquitinated upon EGF-mediated cell activation or upon T-cell costimulation by CD28; which promotes proteasomal degradation.
  • Cellular localization

    Cytoplasmic and Nuclear
  • Database links

  • Alternative names

    • AI429560 antibody
    • AI851073 antibody
    • Cas Br M (murine) ecotropic retroviral transforming sequence b antibody
    • Casitas B lineage lymphoma b antibody
    • Casitas B lineage lymphoma proto oncogene b antibody
    • Casitas B-lineage lymphoma proto-oncogene b antibody
    • Cbl b antibody
    • CBLB antibody
    • CBLB_HUMAN antibody
    • DKFZp686J10223 antibody
    • DKFZp779A0729 antibody
    • DKFZp779F1443 antibody
    • E3 ubiquitin protein ligase CBL B antibody
    • E3 ubiquitin-protein ligase CBL-B antibody
    • EC 6.3.2.- antibody
    • FLJ36865 antibody
    • FLJ41152 antibody
    • Nbla00127 antibody
    • RING finger protein 56 antibody
    • RNF56 antibody
    • SH3 binding protein CBL B antibody
    • SH3-binding protein CBL-B antibody
    • Signal transduction protein CBL B antibody
    • Signal transduction protein CBL-B antibody
    see all


  • Anti-CBLB antibody [246C5a] (ab54362) + immunizing recombinant protein

    Predicted band size: 109 kDa
    Observed band size: 37 kDa
    why is the actual band size different from the predicted?

    The molecular weight of the band on the Western blot does not correspond to the molecular weight of the natural protein as the immunizing recombinant protein fragment was used as the test antigen.
  • IHC image of ab54362 staining in humn T-cell lymphoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab54362, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.


This product has been referenced in:

  • Singh TP  et al. Cbl-b deficiency provides protection against UVB-induced skin damage by modulating inflammatory gene signature. Cell Death Dis 9:835 (2018). Read more (PubMed: 30082827) »
  • Wallner S  et al. The role of the e3 ligase cbl-B in murine dendritic cells. PLoS One 8:e65178 (2013). WB ; Mouse . Read more (PubMed: 23762309) »
See all 2 Publications for this product

Customer reviews and Q&As

Western blot
Mouse Cell lysate - whole cell (mouse embryonic stem cells)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
1e+007 cells
mouse embryonic stem cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Miss. Fabiana Luise

Verified customer

Submitted Nov 17 2015

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