Overview

  • Product name

  • Description

    Rabbit polyclonal to CBLB
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide within Human CBLB aa 90-140 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Sequence:

    SKYDDNQKLAQ LSENEYFKIY IDSLMKKSKR AIRLFKEGKE RMYEEQSQDR


    Database link: Q13191

  • Positive control

    • Human lung carcinoma and rat lung tissues.

Properties

Applications

Our Abpromise guarantee covers the use of ab215991 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500.

Target

  • Relevance

    Function: E3 ubiquitin-protein ligase which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and transfers it to substrates, generally promoting their degradation by the proteasome. Negatively regulates TCR (T-cell receptor), BCR (B-cell receptor) and FCER1 (high affinity immunoglobulin epsilon receptor) signal transduction pathways. In naive T-cells, inhibits VAV1 activation upon TCR engagement and imposes a requirement for CD28 costimulation for proliferation and IL-2 production. Also acts by promoting PIK3R1/p85 ubiquitination, which impairs its recruitment to the TCR and subsequent activation. In activated T-cells, inhibits PLCG1 activation and calcium mobilization upon restimulation and promotes anergy. In B-cells, acts by ubiquitinating SYK and promoting its proteasomal degradation. Slightly promotes SRC ubiquitination. May be involved in EGFR ubiquitination and internalization. May be functionally coupled with the E2 ubiquitin-protein ligase UB2D3. Tissue specificity: Expressed in placenta, heart, lung, kidney, spleen, ovary and testis, as well as fetal brain and liver and hematopoietic cell lines, but not in adult brain, liver, pancreas, salivary gland, or skeletal muscle. Present in lymphocytes (at protein level). Pathway: Protein modification; protein ubiquitination. Similarity: Contains 1 Cbl-PTB (Cbl-type phosphotyrosine-binding) domain. Contains 1 RING-type zinc finger. Contains 1 UBA domain. Domain: The N-terminus is composed of the phosphotyrosine binding (PTB) domain, a short linker region and the RING-type zinc finger. The PTB domain, which is also called TKB (tyrosine kinase binding) domain, is composed of three different subdomains: a four-helix bundle (4H), a calcium-binding EF hand and a divergent SH2 domain. The RING-type zinc finger domain mediates binding to an E2 ubiquitin-conjugating enzyme. The UBA domain interacts with poly-ubiquitinated proteins. PTM: Phosphorylated on tyrosine and serine residues upon TCR or BCR activation, and upon various types of cell stimulation. Auto-ubiquitinated upon EGF-mediated cell activation or upon T-cell costimulation by CD28; which promotes proteasomal degradation.
  • Cellular localization

    Cytoplasmic and Nuclear
  • Database links

  • Alternative names

    • AI429560 antibody
    • AI851073 antibody
    • Cas Br M (murine) ecotropic retroviral transforming sequence b antibody
    • Casitas B lineage lymphoma b antibody
    • Casitas B lineage lymphoma proto oncogene b antibody
    • Casitas B-lineage lymphoma proto-oncogene b antibody
    • Cbl b antibody
    • CBLB antibody
    • CBLB_HUMAN antibody
    • DKFZp686J10223 antibody
    • DKFZp779A0729 antibody
    • DKFZp779F1443 antibody
    • E3 ubiquitin protein ligase CBL B antibody
    • E3 ubiquitin-protein ligase CBL-B antibody
    • EC 6.3.2.- antibody
    • FLJ36865 antibody
    • FLJ41152 antibody
    • Nbla00127 antibody
    • RING finger protein 56 antibody
    • RNF56 antibody
    • SH3 binding protein CBL B antibody
    • SH3-binding protein CBL-B antibody
    • Signal transduction protein CBL B antibody
    • Signal transduction protein CBL-B antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed and paraffin-embedded rat lung tissue labeling CBLB with ab215991 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.

  • Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human lung carcinoma tissue labeling CBLB with ab215991 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.

References

ab215991 has not yet been referenced specifically in any publications.

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