Overview

  • Product name

  • Description

    Rabbit polyclonal to CBLN1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human CBLN1 (internal sequence). A 15 amino acid synthetic peptide from the middle of human CBLN1.

  • Positive control

    • Human prostate tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab64184 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2 - 4 µg/ml. Predicted molecular weight: 21 kDa.
IHC-P Use a concentration of 10 µg/ml.

Target

  • Function

    Required for synapse integrity and synaptic plasticity. During cerebellar synapse formation, essential for the formation and maintenance of parallel fiber and Purkinje cell synapses. When parallel fibers make contact with Purkinje spines, CBLN1 interaction with GRID2 triggers the recruitment of NRXN1 and secretory vesicles to the sites of contact. NRXN1-CBLN1-GRID2 signaling induces presynaptic morphological changes, which may further accumulate pre- and postsynaptic components to promote bidirectional maturation of parallel fiber - Purkinje cell functionnally active synapses by a positive feedback mechanism. Required for CBLN3 export from the endoplasmic reticulum and secretion.
    The cerebellin peptide exerts neuromodulatory functions. Directly stimulates norepinephrine release via the adenylate cyclase/PKA-dependent signaling pathway; and indirectly enhances adrenocortical secretion in vivo, through a paracrine mechanism involving medullary catecholamine release.
  • Tissue specificity

    In the Purkinje cells postsynaptic structures. In the cerebellum, cerebellin is much less abundant than [des-Ser1]-cerebellin.
  • Sequence similarities

    Contains 1 C1q domain.
  • Developmental stage

    Low at birth, the cerebellin concentration increases between day 5 and 15, and reaches peak values between day 21 and 56.
  • Post-translational
    modifications

    The proteolytic processing to yield cerebellin seems to occur either prior to the secretion by presynaptic neurons and subsequent oligomerization or in some other location after release of the mature protein.
  • Cellular localization

    Secreted. Cell junction > synapse > postsynaptic cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • [des-Ser1]-cerebellin antibody
    • CBLN 1 antibody
    • CBLN1 antibody
    • CBLN1_HUMAN antibody
    • CER antibody
    • Cerebellin 1 antibody
    • Cerebellin 1 precursor antibody
    • Cerebellin1 antibody
    • Precerebellin antibody
    see all

Images

  • ab64184 (10ug/ml) staining human CBLN1 in human prostate tissue by immunohistochemistry using formalin-fixed, paraffin-embedded tissue.

References

This product has been referenced in:

  • Rostampour N  et al. Expression of new genes in vertebrate tooth development and p63 signaling. Dev Dyn N/A:N/A (2019). Read more (PubMed: 30875130) »
  • Muguruma K  et al. Ontogeny-recapitulating generation and tissue integration of ES cell-derived Purkinje cells. Nat Neurosci 13:1171-80 (2010). Mouse . Read more (PubMed: 20835252) »
See all 2 Publications for this product

Customer reviews and Q&As

Answer

Thank you for your enquiry.

In answer to your questions:

1. Regarding ab36909 Precerebellin antibody

Based on the immunogen sequence, this antibody should detect precerebellin and cerebellin-1 (precerebellin without the signal peptide), but it should not detect cerebellin or [des-Ser1]-cerebellin.

The immunogen does span the cleavage site for cerebellin, but there does not seem to be enough overlap with the cerebellin peptide sequence to merit antibody cross-reaction.

I am sorry we are not able to provide the exact immunogen sequence on this occasion as this particular antibody is sourced externally and the information is confidential.

Here is a link to the Uniprot protein database site page for cerebellin 1 which will have further information:

http://www.uniprot.org/uniprot/P23435

2. The CBLN1 antibody (ab64184) will detect cerebellin 1.

I hope this information will be helpful to you. If you have any further questions, please do not hesitate to contact us.

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