Overview

  • Product name
    Anti-CBLN1 antibody [EPR13649]
    See all CBLN1 primary antibodies
  • Description
    Rabbit monoclonal [EPR13649] to CBLN1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human CBLN1 aa 50-150 (Cysteine residue). The exact sequence is proprietary.
    Database link: P23435

  • Positive control
    • Human cerebellum, Human fetal brain tissue lysate; Neuro-2a, U87-MG, SH-SY5Y, C6 and Raw 264.7 cell lysates
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR13649
  • Isotype
    IgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab181379 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 16 kDa (predicted molecular weight: 21 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
  • Target

    • Function
      Required for synapse integrity and synaptic plasticity. During cerebellar synapse formation, essential for the formation and maintenance of parallel fiber and Purkinje cell synapses. When parallel fibers make contact with Purkinje spines, CBLN1 interaction with GRID2 triggers the recruitment of NRXN1 and secretory vesicles to the sites of contact. NRXN1-CBLN1-GRID2 signaling induces presynaptic morphological changes, which may further accumulate pre- and postsynaptic components to promote bidirectional maturation of parallel fiber - Purkinje cell functionnally active synapses by a positive feedback mechanism. Required for CBLN3 export from the endoplasmic reticulum and secretion.
      The cerebellin peptide exerts neuromodulatory functions. Directly stimulates norepinephrine release via the adenylate cyclase/PKA-dependent signaling pathway; and indirectly enhances adrenocortical secretion in vivo, through a paracrine mechanism involving medullary catecholamine release.
    • Tissue specificity
      In the Purkinje cells postsynaptic structures. In the cerebellum, cerebellin is much less abundant than [des-Ser1]-cerebellin.
    • Sequence similarities
      Contains 1 C1q domain.
    • Developmental stage
      Low at birth, the cerebellin concentration increases between day 5 and 15, and reaches peak values between day 21 and 56.
    • Post-translational
      modifications
      The proteolytic processing to yield cerebellin seems to occur either prior to the secretion by presynaptic neurons and subsequent oligomerization or in some other location after release of the mature protein.
    • Cellular localization
      Secreted. Cell junction > synapse > postsynaptic cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • [des-Ser1]-cerebellin antibody
      • CBLN 1 antibody
      • CBLN1 antibody
      • CBLN1_HUMAN antibody
      • CER antibody
      • Cerebellin 1 antibody
      • Cerebellin 1 precursor antibody
      • Cerebellin1 antibody
      • Precerebellin antibody
      see all

    Images

    • All lanes : Anti-CBLN1 antibody [EPR13649] (ab181379) at 1/1000 dilution

      Lane 1 : Human cerebellum tissue lysate
      Lane 2 : Human fetal brain tissue lysate
      Lane 3 : Neuro-2a cell lysate
      Lane 4 : U87-MG cell lysate
      Lane 5 : SH-SY5Y cell lysate
      Lane 6 : C6 cell lysate
      Lane 7 : Raw 264.7 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 21 kDa

    References

    This product has been referenced in:
    • zur Nedden S  et al. Protein kinase N1 critically regulates cerebellar development and long-term function. J Clin Invest 128:2076-2088 (2018). Read more (PubMed: 29494346) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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