The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/10000. Detects a band of approximately 16 kDa (predicted molecular weight: 21 kDa).
Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
Required for synapse integrity and synaptic plasticity. During cerebellar synapse formation, essential for the formation and maintenance of parallel fiber and Purkinje cell synapses. When parallel fibers make contact with Purkinje spines, CBLN1 interaction with GRID2 triggers the recruitment of NRXN1 and secretory vesicles to the sites of contact. NRXN1-CBLN1-GRID2 signaling induces presynaptic morphological changes, which may further accumulate pre- and postsynaptic components to promote bidirectional maturation of parallel fiber - Purkinje cell functionnally active synapses by a positive feedback mechanism. Required for CBLN3 export from the endoplasmic reticulum and secretion. The cerebellin peptide exerts neuromodulatory functions. Directly stimulates norepinephrine release via the adenylate cyclase/PKA-dependent signaling pathway; and indirectly enhances adrenocortical secretion in vivo, through a paracrine mechanism involving medullary catecholamine release.
In the Purkinje cells postsynaptic structures. In the cerebellum, cerebellin is much less abundant than [des-Ser1]-cerebellin.
Contains 1 C1q domain.
Low at birth, the cerebellin concentration increases between day 5 and 15, and reaches peak values between day 21 and 56.
The proteolytic processing to yield cerebellin seems to occur either prior to the secretion by presynaptic neurons and subsequent oligomerization or in some other location after release of the mature protein.
Western blot - Anti-CBLN1 antibody [EPR13649] (ab181379)
All lanes : Anti-CBLN1 antibody [EPR13649] (ab181379) at 1/1000 dilution
Lane 1 : Human cerebellum tissue lysate Lane 2 : Human fetal brain tissue lysate Lane 3 : Neuro-2a cell lysate Lane 4 : U87-MG cell lysate Lane 5 : SH-SY5Y cell lysate Lane 6 : C6 cell lysate Lane 7 : Raw 264.7 cell lysate