Overview

  • Product name

    Anti-CBP80 antibody - N-terminal
    See all CBP80 primary antibodies
  • Description

    Rabbit polyclonal to CBP80 - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Chicken
  • Immunogen

    Recombinant fragment within Human CBP80 (N terminal). The exact sequence is proprietary.
    Database link: Q09161

  • Positive control

    • WB: HeLa whole cell lysate. ICC/IF: HeLa cells. IHC-P: Mouse fore brain tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab228885 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 92 kDa.
IHC-P 1/100 - 1/1000.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Component of the cap-binding complex (CBC), which binds co-transcriptionally to the 5' cap of pre-mRNAs and is involved in various processes such as pre-mRNA splicing, translation regulation, nonsense-mediated mRNA decay, RNA-mediated gene silencing (RNAi) by microRNAs (miRNAs) and mRNA export. The CBC complex is involved in mRNA export from the nucleus via its interaction with THOC4/ALY, leading to the recruitment of the mRNA export machinery to the 5' end of mRNA and to mRNA export in a 5' to 3' direction through the nuclear pore. The CBC complex is also involved in mediating U snRNA and intronless mRNAs export from the nucleus. The CBC complex is essential for a pioneer round of mRNA translation, before steady state translation when the CBC complex is replaced by cytoplasmic cap-binding protein eIF4E. The pioneer round of mRNA translation mediated by the CBC complex plays a central role in nonsense-mediated mRNA decay (NMD), NMD only taking place in mRNAs bound to the CBC complex, but not on eIF4E-bound mRNAs. The CBC complex enhances NMD in mRNAs containing at least one exon-junction complex (EJC) via its interaction with UPF1, promoting the interaction between UPF1 and UPF2. The CBC complex is also involved in 'failsafe' NMD, which is independent of the EJC complex, while it does not participate in Staufen-mediated mRNA decay (SMD). During cell proliferation, the CBC complex is also involved in microRNAs (miRNAs) biogenesis via its interaction with SRRT/ARS2 and is required for miRNA-mediated RNA interference. The CBC complex also acts as a negative regulator of PARN, thereby acting as an inhibitor of mRNA deadenylation. In the CBC complex, NCBP1/CBP80 does not bind directly capped RNAs (m7GpppG-capped RNA) but is required to stabilize the movement of the N-terminal loop of NCBP2/CBP20 and lock the CBC into a high affinity cap-binding state with the cap structure.
  • Sequence similarities

    Belongs to the NCBP1 family.
    Contains 1 MIF4G domain.
  • Cellular localization

    Nucleus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
  • Information by UniProt
  • Database links

  • Alternative names

    • 80 kDa nuclear cap-binding protein antibody
    • CBP80 antibody
    • MGC2087 antibody
    • NCBP 80 kDa subunit antibody
    • NCBP antibody
    • ncbp1 antibody
    • NCBP1_HUMAN antibody
    • nuclear cap binding protein subunit 1 antibody
    • nuclear cap binding protein subunit 1, 80kDa antibody
    • Nuclear cap-binding protein subunit 1 antibody
    • Sto1 antibody
    see all

Images

  • Anti-CBP80 antibody - N-terminal (ab228885) at 1/1000 dilution + HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 30 µg

    Developed using the ECL technique.

    Predicted band size: 92 kDa



    7.5% SDS-PAGE gel.

  • Paraffin-embedded mouse fore brain tissue stained for CBP80 using ab228885 at 1/500 dilution in immunohistochemical analysis.

  • Paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for CBP80 (green) using ab228885 at 1/200 dilution in ICC/IF.

    Nuclear counterstain: Hoechst 33342 (blue)

References

ab228885 has not yet been referenced specifically in any publications.

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