Anti-CBR1 antibody (ab186825)
Key features and details
- Rabbit polyclonal to CBR1
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-CBR1 antibody
See all CBR1 primary antibodies -
Description
Rabbit polyclonal to CBR1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant full length protein corresponding to Human CBR1 aa 1-277.
Database link: P16152 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 49% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab186825 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/2000. Predicted molecular weight: 30 kDa.
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Notes |
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WB
1/500 - 1/2000. Predicted molecular weight: 30 kDa. |
Target
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Function
NADPH-dependent reductase with broad substrate specificity. Catalyzes the reduction of a wide variety of carbonyl compounds including quinones, prostaglandins, menadione, plus various xenobiotics. Catalyzes the reduction of the antitumor anthracyclines doxorubicin and daunorubicin to the cardiotoxic compounds doxorubicinol and daunorubicinol. Can convert prostaglandin E2 to prostaglandin F2-alpha. Can bind glutathione, which explains its higher affinity for glutathione-conjugated substrates. Catalyzes the reduction of S-nitrosoglutathione. -
Sequence similarities
Belongs to the short-chain dehydrogenases/reductases (SDR) family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 873 Human
- Entrez Gene: 12408 Mouse
- Omim: 114830 Human
- SwissProt: P16152 Human
- SwissProt: P48758 Mouse
- Unigene: 606200 Human
- Unigene: 88778 Human
- Unigene: 26940 Mouse
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Alternative names
- 15 hydroxyprostaglandin dehydrogenase [NADP+] antibody
- 15-hydroxyprostaglandin dehydrogenase [NADP+] antibody
- Carbonyl reductase [NADPH] 1 antibody
see all
Images
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All lanes : Anti-CBR1 antibody (ab186825) at 1/500 dilution
Lane 1 : SW480 cell line extract
Lane 2 : 22RV1 cell line extract
Lane 3 : MCF7 cell line extract
Lane 4 : H460 cell line extract
Lane 5 : SKOV3 cell line extract
Lane 6 : Mouse kidney cell line extract
Lane 7 : Mouse liver cell line extract
Lane 8 : Mouse lung cell line extract
Lane 9 : Mouse testis cell line extract
Predicted band size: 30 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab186825 has been referenced in 3 publications.
- Zheng J et al. Maternal 25-Hydroxyvitamin D Deficiency Promoted Metabolic Syndrome and Downregulated Nrf2/CBR1 Pathway in Offspring. Front Pharmacol 11:97 (2020). PubMed: 32184720
- Jiang Q et al. Flufenamic acid alleviates sepsis-induced lung injury by up-regulating CBR1. Drug Dev Res 81:885-892 (2020). PubMed: 32542754
- Kwon JH et al. Upregulation of Carbonyl Reductase 1 by Nrf2 as a Potential Therapeutic Intervention for Ischemia/ Reperfusion Injury during Liver Transplantation. Mol Cells 42:672-685 (2019). PubMed: 31486328