Key features and details
- Rabbit polyclonal to CBR1
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-CBR1 antibody
See all CBR1 primary antibodies
DescriptionRabbit polyclonal to CBR1
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Recombinant full length protein corresponding to Human CBR1 aa 1-277.
Database link: P16152
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 49% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab186825 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2000. Predicted molecular weight: 30 kDa.|
FunctionNADPH-dependent reductase with broad substrate specificity. Catalyzes the reduction of a wide variety of carbonyl compounds including quinones, prostaglandins, menadione, plus various xenobiotics. Catalyzes the reduction of the antitumor anthracyclines doxorubicin and daunorubicin to the cardiotoxic compounds doxorubicinol and daunorubicinol. Can convert prostaglandin E2 to prostaglandin F2-alpha. Can bind glutathione, which explains its higher affinity for glutathione-conjugated substrates. Catalyzes the reduction of S-nitrosoglutathione.
Sequence similaritiesBelongs to the short-chain dehydrogenases/reductases (SDR) family.
- Information by UniProt
- 15 hydroxyprostaglandin dehydrogenase [NADP+] antibody
- 15-hydroxyprostaglandin dehydrogenase [NADP+] antibody
- Carbonyl reductase [NADPH] 1 antibody
All lanes : Anti-CBR1 antibody (ab186825) at 1/500 dilution
Lane 1 : SW480 cell line extract
Lane 2 : 22RV1 cell line extract
Lane 3 : MCF7 cell line extract
Lane 4 : H460 cell line extract
Lane 5 : SKOV3 cell line extract
Lane 6 : Mouse kidney cell line extract
Lane 7 : Mouse liver cell line extract
Lane 8 : Mouse lung cell line extract
Lane 9 : Mouse testis cell line extract
Predicted band size: 30 kDa
ab186825 has been referenced in 2 publications.
- Zheng J et al. Maternal 25-Hydroxyvitamin D Deficiency Promoted Metabolic Syndrome and Downregulated Nrf2/CBR1 Pathway in Offspring. Front Pharmacol 11:97 (2020). PubMed: 32184720
- Kwon JH et al. Upregulation of Carbonyl Reductase 1 by Nrf2 as a Potential Therapeutic Intervention for Ischemia/ Reperfusion Injury during Liver Transplantation. Mol Cells 42:672-685 (2019). PubMed: 31486328