Western blot abreview for Anti-CBR3 antibody

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Abreviews
Application
Western blot
Sample
Human Recombinant protein (Recombinant protein)
Loading amount
0.03 µg
Specification
Recombinant protein
Gel Running Conditions
Non-reduced Denaturing (12% Gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Dilution
1/1000
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: PBS-tween (0.05%)

Secondary antibody

Name
Non-Abcam antibody was used: Anti-Goat IgG (whole molecule)
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000

Detection

Detection method
ECL
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 30 kDa
Positive control
Recombinant CBR3 protein (ab86848) was loaded in increasing amounts to create a standard curve for protein quantification in our samples of interest (various human tissue).

Additional data

Additional Notes
Nitrocellulose membranes were used in our WB. The image of the standard curve shows the CBR3 recombinant protein (ab86848) in increasing amounts from left to right (0.03, 0.07, and 0.1 ug) and a protein ladder on the far right as detected using the conditions outlined above.

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Submitted Oct 07 2011

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