Recombinant
RabMAb

Recombinant Anti-CBR3 antibody [EPR14129] (ab180939)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14129] to CBR3
  • Suitable for: Flow Cyt, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-CBR3 antibody [EPR14129]
    See all CBR3 primary antibodies

  • Description

    Rabbit monoclonal [EPR14129] to CBR3

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human CBR3 aa 150-250. The exact sequence is proprietary.
    Database link: O75828

  • Positive control

    • HeLa, K562 and 293 cell lysate. K562 cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab180939 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/130.
WB 1/10000 - 1/50000. Predicted molecular weight: 30 kDa.

Target

  • Function

    Has low NADPH-dependent oxidoreductase activity towards 4-benzoylpyridine and menadione (in vitro).

  • Tissue specificity

    Detected in ovary, pancreas, intestine, colon, kidney, brain, thymus, lung, heart, liver, spleen, leukocyte, prostate and testis.

  • Sequence similarities

    Belongs to the short-chain dehydrogenases/reductases (SDR) family.

  • Cellular localization

    Cytoplasm.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • Carbonyl reductase (NADPH) 3 antibody
    • Carbonyl reductase (NADPH) 3 EC 1.1.1.184 antibody
    • Carbonyl reductase [NADPH] 3 antibody
    • Carbonyl reductase 3 antibody
    • CBR 3 antibody
    • CBR3 antibody
    • CBR3_HUMAN antibody
    • EC 1.1.1.184 antibody
    • Epididymis secretory protein Li 25 antibody
    • hCBR3 antibody
    • HEL S 25 antibody
    • NADPH dependent carbonyl reductase 3 antibody
    • NADPH-dependent carbonyl reductase 3 antibody
    • SDR21C2 antibody
    • Short chain dehydrogenase/reductase family 21C member 2 antibody
    see all

Images

  • Western blot - Anti-CBR3 antibody [EPR14129] (ab180939)
    Western blot - Anti-CBR3 antibody [EPR14129] (ab180939)
    All lanes : Anti-CBR3 antibody [EPR14129] (ab180939) at 1/20000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : K562 cell lysate
    Lane 3 : 293 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 30 kDa



    Blocking/ Dilution buffer: 5% NFDM /TBST.

  • Flow Cytometry - Anti-CBR3 antibody [EPR14129] (ab180939)
    Flow Cytometry - Anti-CBR3 antibody [EPR14129] (ab180939)

    Flow Cytometry analysis of K562 cells labeling CBR3 using ab180939 at a 1/130 dilution (pink). Goat anti rabbit IgG (FITC) used as the secondary at a 1/150 dilution. Isotype control Rabbit monoclonal IgG (green). Cells were fixed in 2% paraformaldehyde.

References

Publishing research using ab180939? Please let us know so that we can cite the reference in this datasheet.

ab180939 has not yet been referenced specifically in any publications.

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