Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14129] to CBR3
- Suitable for: Flow Cyt, WB
- Reacts with: Mouse, Rat, Human
Product nameAnti-CBR3 antibody [EPR14129]
See all CBR3 primary antibodies
DescriptionRabbit monoclonal [EPR14129] to CBR3
Tested applicationsSuitable for: Flow Cyt, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human CBR3 aa 150-250. The exact sequence is proprietary.
Database link: O75828
- HeLa, K562 and 293 cell lysate. K562 cells.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab180939 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Predicted molecular weight: 30 kDa.|
FunctionHas low NADPH-dependent oxidoreductase activity towards 4-benzoylpyridine and menadione (in vitro).
Tissue specificityDetected in ovary, pancreas, intestine, colon, kidney, brain, thymus, lung, heart, liver, spleen, leukocyte, prostate and testis.
Sequence similaritiesBelongs to the short-chain dehydrogenases/reductases (SDR) family.
- Information by UniProt
- Carbonyl reductase (NADPH) 3 antibody
- Carbonyl reductase (NADPH) 3 EC 220.127.116.11 antibody
- Carbonyl reductase [NADPH] 3 antibody
All lanes : Anti-CBR3 antibody [EPR14129] (ab180939) at 1/20000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : K562 cell lysate
Lane 3 : 293 cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 30 kDa
Blocking/ Dilution buffer: 5% NFDM /TBST.
Flow Cytometry analysis of K562 cells labeling CBR3 using ab180939 at a 1/130 dilution (pink). Goat anti rabbit IgG (FITC) used as the secondary at a 1/150 dilution. Isotype control Rabbit monoclonal IgG (green). Cells were fixed in 2% paraformaldehyde.
ab180939 has not yet been referenced specifically in any publications.