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    cbr3-antibody-epr14129-ab180939.pdf

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Signal Transduction Metabolism Alcohol metabolism
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RecombinantRabMAb

Recombinant Anti-CBR3 antibody [EPR14129] (ab180939)

  • Datasheet
  • SDS
  • Certificate of Compliance
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Western blot - Anti-CBR3 antibody [EPR14129] (ab180939)
  • Flow Cytometry (Intracellular) - Anti-CBR3 antibody [EPR14129] (ab180939)
  • Anti-CBR3 antibody [EPR14129] (ab180939)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14129] to CBR3
  • Suitable for: Flow Cyt (Intra), WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

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Secondary
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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Primary
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Anti-CBR3 antibody [EPR14129] - BSA and Azide free (ab250271)
Protein
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Recombinant Human CBR3 protein (ab86848)

View more associated products

Overview

  • Product name

    Anti-CBR3 antibody [EPR14129]
    See all CBR3 primary antibodies
  • Description

    Rabbit monoclonal [EPR14129] to CBR3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt (Intra), WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HeLa, K562 and 293 cell lysate. K562 cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR14129
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Metabolism
    • Alcohol metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Alcohol metabolism

Associated products

  • Alternative Versions

    • Anti-CBR3 antibody [EPR14129] - BSA and Azide free (ab250271)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)
    • K-562 whole cell lysate (ab7911)
  • Recombinant Protein

    • Recombinant Human CBR3 protein (ab86848)
  • Related Products

    • Recombinant Human CBR3 protein (ab86848)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab180939 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/130.
WB
1/10000 - 1/50000. Predicted molecular weight: 30 kDa.
Notes
Flow Cyt (Intra)
1/130.
WB
1/10000 - 1/50000. Predicted molecular weight: 30 kDa.

Target

  • Function

    Has low NADPH-dependent oxidoreductase activity towards 4-benzoylpyridine and menadione (in vitro).
  • Tissue specificity

    Detected in ovary, pancreas, intestine, colon, kidney, brain, thymus, lung, heart, liver, spleen, leukocyte, prostate and testis.
  • Sequence similarities

    Belongs to the short-chain dehydrogenases/reductases (SDR) family.
  • Cellular localization

    Cytoplasm.
  • Target information above from: UniProt accession O75828 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 874 Human
    • Entrez Gene: 109857 Mouse
    • Entrez Gene: 304078 Rat
    • Omim: 603608 Human
    • SwissProt: O75828 Human
    • SwissProt: Q8K354 Mouse
    • Unigene: 154510 Human
    • Unigene: 4512 Mouse
    • Alternative names

      • Carbonyl reductase (NADPH) 3 antibody
      • Carbonyl reductase (NADPH) 3 EC 1.1.1.184 antibody
      • Carbonyl reductase [NADPH] 3 antibody
      • Carbonyl reductase 3 antibody
      • CBR 3 antibody
      • CBR3 antibody
      • CBR3_HUMAN antibody
      • EC 1.1.1.184 antibody
      • Epididymis secretory protein Li 25 antibody
      • hCBR3 antibody
      • HEL S 25 antibody
      • NADPH dependent carbonyl reductase 3 antibody
      • NADPH-dependent carbonyl reductase 3 antibody
      • SDR21C2 antibody
      • Short chain dehydrogenase/reductase family 21C member 2 antibody
      see all

    Images

    • Western blot - Anti-CBR3 antibody [EPR14129] (ab180939)
      Western blot - Anti-CBR3 antibody [EPR14129] (ab180939)
      All lanes : Anti-CBR3 antibody [EPR14129] (ab180939) at 1/20000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : K562 cell lysate
      Lane 3 : 293 cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 30 kDa



      Blocking/ Dilution buffer: 5% NFDM /TBST.

    • Flow Cytometry (Intracellular) - Anti-CBR3 antibody [EPR14129] (ab180939)
      Flow Cytometry (Intracellular) - Anti-CBR3 antibody [EPR14129] (ab180939)

      Intracellular Flow Cytometry analysis of K562 cells labeling CBR3 using ab180939 at a 1/130 dilution (pink). Goat anti rabbit IgG (FITC) used as the secondary at a 1/150 dilution. Isotype control Rabbit monoclonal IgG (green). Cells were fixed in 2% paraformaldehyde. 

       

       

    • Anti-CBR3 antibody [EPR14129] (ab180939)
      Anti-CBR3 antibody [EPR14129] (ab180939)

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (0)

    Publishing research using ab180939? Please let us know so that we can cite the reference in this datasheet.

    ab180939 has not yet been referenced specifically in any publications.

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