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RecombinantRabMAb

Recombinant Anti-CBR3 antibody [EPR14129] (ab180939)

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Western blot - Anti-CBR3 antibody [EPR14129] (ab180939)
  • Flow Cytometry (Intracellular) - Anti-CBR3 antibody [EPR14129] (ab180939)
  • Anti-CBR3 antibody [EPR14129] (ab180939)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14129] to CBR3
  • Suitable for: Flow Cyt (Intra), WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-CBR3 antibody [EPR14129]
    See all CBR3 primary antibodies

  • Description

    Rabbit monoclonal [EPR14129] to CBR3

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), WBmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HeLa, K562 and 293 cell lysate. K562 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab180939 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/130.
WB
1/10000 - 1/50000. Predicted molecular weight: 30 kDa.
Notes
Flow Cyt (Intra)
1/130.
WB
1/10000 - 1/50000. Predicted molecular weight: 30 kDa.

Target

  • Function

    Has low NADPH-dependent oxidoreductase activity towards 4-benzoylpyridine and menadione (in vitro).

  • Tissue specificity

    Detected in ovary, pancreas, intestine, colon, kidney, brain, thymus, lung, heart, liver, spleen, leukocyte, prostate and testis.

  • Sequence similarities

    Belongs to the short-chain dehydrogenases/reductases (SDR) family.

  • Cellular localization

    Cytoplasm.
  • Information by UniProt

  • Database links

    • Alternative names

      • Carbonyl reductase (NADPH) 3 antibody
      • Carbonyl reductase (NADPH) 3 EC 1.1.1.184 antibody
      • Carbonyl reductase [NADPH] 3 antibody
      • Carbonyl reductase 3 antibody
      • CBR 3 antibody
      • CBR3 antibody
      • CBR3_HUMAN antibody
      • EC 1.1.1.184 antibody
      • Epididymis secretory protein Li 25 antibody
      • hCBR3 antibody
      • HEL S 25 antibody
      • NADPH dependent carbonyl reductase 3 antibody
      • NADPH-dependent carbonyl reductase 3 antibody
      • SDR21C2 antibody
      • Short chain dehydrogenase/reductase family 21C member 2 antibody
      see all

    Images

    • Western blot - Anti-CBR3 antibody [EPR14129] (ab180939)
      Western blot - Anti-CBR3 antibody [EPR14129] (ab180939)
      All lanes : Anti-CBR3 antibody [EPR14129] (ab180939) at 1/20000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : K562 cell lysate
      Lane 3 : 293 cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 30 kDa



      Blocking/ Dilution buffer: 5% NFDM /TBST.

    • Flow Cytometry (Intracellular) - Anti-CBR3 antibody [EPR14129] (ab180939)
      Flow Cytometry (Intracellular) - Anti-CBR3 antibody [EPR14129] (ab180939)

      Intracellular Flow Cytometry analysis of K562 cells labeling CBR3 using ab180939 at a 1/130 dilution (pink). Goat anti rabbit IgG (FITC) used as the secondary at a 1/150 dilution. Isotype control Rabbit monoclonal IgG (green). Cells were fixed in 2% paraformaldehyde. 

       

       

    • Anti-CBR3 antibody [EPR14129] (ab180939)
      Anti-CBR3 antibody [EPR14129] (ab180939)

    References (0)

    Publishing research using ab180939? Please let us know so that we can cite the reference in this datasheet.

    ab180939 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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