Overview

  • Product name

  • Description

    Rabbit polyclonal to CBS
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to a region within internal sequence amino acids 308-338 of Human CBS

  • Positive control

    • Mouse kidney tissue lysates, Human brain tissue and 293 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab135626 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 61 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

ICC/IF 1/10 - 1/50.

Target

  • Function

    Only known pyridoxal phosphate-dependent enzyme that contains heme. Important regulator of hydrogen sulfide, especially in the brain, utilizing cysteine instead of serine to catalyze the formation of hydrogen sulfide. Hydrogen sulfide is a gastratransmitter with signaling and cytoprotective effects such as acting as a neuromodulator in the brain to protect neurons against hypoxic injury.
  • Tissue specificity

    In the adult strongly expressed in liver and pancreas, some expression in heart and brain, weak expression in lung and kidney. In the fetus, expressed in brain, liver and kidney.
  • Pathway

    Amino-acid biosynthesis; L-cysteine biosynthesis; L-cysteine from L-homocysteine and L-serine: step 1/2.
  • Involvement in disease

    Defects in CBS are the cause of cystathionine beta-synthase deficiency (CBSD) [MIM:236200]. CBSD is an enzymatic deficiency resulting in altered sulfur metabolism and homocystinuria. The clinical features of untreated homocystinuria due to CBS deficiency include myopia, ectopia lentis, mental retardation, skeletal anomalies resembling Marfan syndrome, and thromboembolic events. Light skin and hair can also be present. Biochemical features include increased urinary homocystine and methionine.
  • Sequence similarities

    Belongs to the cysteine synthase/cystathionine beta-synthase family.
    Contains 1 CBS domain.
  • Cellular localization

    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • AI047524 antibody
    • AI303044 antibody
    • Beta thionase antibody
    • Beta-thionase antibody
    • Cbs antibody
    • Cbs cystathionine beta-synthase antibody
    • CBS_HUMAN antibody
    • Cystathionine beta synthase antibody
    • Cystathionine beta-synthase antibody
    • EC 4.2.1.22 antibody
    • HIP 4 antibody
    • HIP4 antibody
    • Methylcysteine synthase antibody
    • MGC18856 antibody
    • MGC18895 antibody
    • MGC37300 antibody
    • OTTHUMP00000109416 antibody
    • OTTHUMP00000109418 antibody
    • Serine sulfhydrase antibody
    see all

Images

  • Anti-CBS antibody (ab135626) at 1/100 dilution + Mouse kidney tissue lysate at 35 µg

    Predicted band size: 61 kDa

  • All lanes : Anti-CBS antibody (ab135626) at 1/100 dilution

    Lane 1 : nontransfected 293 cells lysate
    Lane 2 : lysate of 293 cells transiently transfected with the CBS gene

    Lysates/proteins at 2 µg per lane.

    Predicted band size: 61 kDa

  • Immunohistochemical analysis of Formalin-fixed, Paraffin-embedded Human brain tissue labelling CBS with ab135626 at 1/50 dilution followed by peroxidase-conjugated secondary antibody and DAB staining.
  • Flow cytometric analysis of ab135626 staining CBS at 1/10 in 293 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
  • Confocal immunofluorescent analysis of CBS using ab135626 at 1/10 dilution in 293 cell followed by Alexa Fluor 488 conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).

References

This product has been referenced in:

  • Xu Y  et al. Dietary methionine restriction ameliorates the impairment of learning and memory function induced by obesity in mice. Food Funct 10:1411-1425 (2019). Read more (PubMed: 30758370) »
  • Lin Z  et al. Hydrogen sulfide attenuates oxidative stress-induced NLRP3 inflammasome activation via S-sulfhydrating c-Jun at Cys269 in macrophages. Biochim Biophys Acta N/A:N/A (2018). WB ; Human . Read more (PubMed: 29859240) »
See all 6 Publications for this product

Customer reviews and Q&As

Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (breast (MCF7)and lung (A459) cancer cell line)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
25 µg
Specification
breast (MCF7)and lung (A459) cancer cell line
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jun 30 2016

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