Overview

  • Product name

  • Description

    Rabbit polyclonal to CBS
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human CBS aa 1-50. The exact sequence is proprietary.
    Database link: P35520

  • Positive control

    • WB: HeLa and HEK-293T whole cell lysates. IP: HEK-293T whole cell lysate. IHC-P: Human ovarian carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab226194 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-10 µg/mg of lysate.
IHC-P 1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Predicted molecular weight: 61,62 kDa.

Target

  • Function

    Only known pyridoxal phosphate-dependent enzyme that contains heme. Important regulator of hydrogen sulfide, especially in the brain, utilizing cysteine instead of serine to catalyze the formation of hydrogen sulfide. Hydrogen sulfide is a gastratransmitter with signaling and cytoprotective effects such as acting as a neuromodulator in the brain to protect neurons against hypoxic injury.
  • Tissue specificity

    In the adult strongly expressed in liver and pancreas, some expression in heart and brain, weak expression in lung and kidney. In the fetus, expressed in brain, liver and kidney.
  • Pathway

    Amino-acid biosynthesis; L-cysteine biosynthesis; L-cysteine from L-homocysteine and L-serine: step 1/2.
  • Involvement in disease

    Defects in CBS are the cause of cystathionine beta-synthase deficiency (CBSD) [MIM:236200]. CBSD is an enzymatic deficiency resulting in altered sulfur metabolism and homocystinuria. The clinical features of untreated homocystinuria due to CBS deficiency include myopia, ectopia lentis, mental retardation, skeletal anomalies resembling Marfan syndrome, and thromboembolic events. Light skin and hair can also be present. Biochemical features include increased urinary homocystine and methionine.
  • Sequence similarities

    Belongs to the cysteine synthase/cystathionine beta-synthase family.
    Contains 1 CBS domain.
  • Cellular localization

    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • AI047524 antibody
    • AI303044 antibody
    • Beta thionase antibody
    • Beta-thionase antibody
    • Cbs antibody
    • Cbs cystathionine beta-synthase antibody
    • CBS_HUMAN antibody
    • Cystathionine beta synthase antibody
    • Cystathionine beta-synthase antibody
    • EC 4.2.1.22 antibody
    • HIP 4 antibody
    • HIP4 antibody
    • Methylcysteine synthase antibody
    • MGC18856 antibody
    • MGC18895 antibody
    • MGC37300 antibody
    • OTTHUMP00000109416 antibody
    • OTTHUMP00000109418 antibody
    • Serine sulfhydrase antibody
    see all

Images

  • All lanes : Anti-CBS antibody (ab226194) at 0.1 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 61,62 kDa


    Exposure time: 30 seconds


    Cell lysates were prepared in NETN lysis buffer.

  • Formalin-fixed, paraffin-embedded human ovarian carcinoma tissue stained for CBS using ab226194 at 1/5000 dilution in immunohistochemical analysis. Detection: DAB staining.

  • CBS was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab226194 at 6 µg per reaction. Western blot was performed from the immunoprecipitate using ab226194 at 0.4 µg/ml.

    Lane 1: ab226194 IP in HEK-293T whole cell lysate.

    Lane 2: Control IgG IP in HEK-293T whole cell lysate.

    Detection: Chemiluminescence with exposure time of 30 seconds.

References

ab226194 has not yet been referenced specifically in any publications.

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