Anti-CBS antibody (ab96252)
Key features and details
- Rabbit polyclonal to CBS
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human, Pig, Zebrafish
- Isotype: IgG
Overview
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Product name
Anti-CBS antibody
See all CBS primary antibodies -
Description
Rabbit polyclonal to CBS -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human, Pig, Zebrafish
Predicted to work with: Mouse, Rat, Rabbit, Cow -
Immunogen
Recombinant fragment corresponding to a region within amino acids 39-221 of Human CBS (AAH00440).
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Positive control
- WB: HeLa, Molt-4 and Raji whole cell lysates IHC-P: OVCAR3 xenograft; ICC: HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab96252 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 5 µg/ml.
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WB |
1/500 - 1/3000. Predicted molecular weight: 61 kDa.
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IHC-P |
1/100 - 1/1000.
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Notes |
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ICC/IF
Use a concentration of 5 µg/ml. |
WB
1/500 - 1/3000. Predicted molecular weight: 61 kDa. |
IHC-P
1/100 - 1/1000. |
Target
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Function
Only known pyridoxal phosphate-dependent enzyme that contains heme. Important regulator of hydrogen sulfide, especially in the brain, utilizing cysteine instead of serine to catalyze the formation of hydrogen sulfide. Hydrogen sulfide is a gastratransmitter with signaling and cytoprotective effects such as acting as a neuromodulator in the brain to protect neurons against hypoxic injury. -
Tissue specificity
In the adult strongly expressed in liver and pancreas, some expression in heart and brain, weak expression in lung and kidney. In the fetus, expressed in brain, liver and kidney. -
Pathway
Amino-acid biosynthesis; L-cysteine biosynthesis; L-cysteine from L-homocysteine and L-serine: step 1/2. -
Involvement in disease
Defects in CBS are the cause of cystathionine beta-synthase deficiency (CBSD) [MIM:236200]. CBSD is an enzymatic deficiency resulting in altered sulfur metabolism and homocystinuria. The clinical features of untreated homocystinuria due to CBS deficiency include myopia, ectopia lentis, mental retardation, skeletal anomalies resembling Marfan syndrome, and thromboembolic events. Light skin and hair can also be present. Biochemical features include increased urinary homocystine and methionine. -
Sequence similarities
Belongs to the cysteine synthase/cystathionine beta-synthase family.
Contains 1 CBS domain. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 875 Human
- Entrez Gene: 12411 Mouse
- Entrez Gene: 24250 Rat
- Entrez Gene: 550524 Zebrafish
- Omim: 613381 Human
- SwissProt: P35520 Human
- SwissProt: Q91WT9 Mouse
- SwissProt: P32232 Rat
see all -
Alternative names
- AI047524 antibody
- AI303044 antibody
- Beta thionase antibody
see all
Images
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All lanes : Anti-CBS antibody (ab96252) at 1/5000 dilution
Lane 1 : WT HeLa cell extract
Lane 2 : CBS Knockout HeLa cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 61 kDa -
All lanes : Anti-CBS antibody (ab96252) at 1/5000 dilution
Lane 1 : Molt-4 whole cell lysate
Lane 2 : Raji whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 61 kDa
7.5% SDS PAGE -
Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft using ab96252 at 1/500 dilution.
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All lanes : Anti-CBS antibody (ab96252) at 1 µg/ml
Lane 1 : Marker
Lane 2 : Zebrafish brain homogenate at 20 µg
Lane 3 : Zebrafish heart homogenate at 20 µg
Lane 4 : Zebrafish liver homogenate at 20 µg
Lane 5 : Human epithelial carcinoma cell line at 20 µg
Secondary
All lanes : Goat polyclonal to Rabbit IgG – H&L – Pre-Adsorbed (HRP) at 1/6000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 61 kDa
Observed band size: 72 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes -
HeLa cells stained for CBS (green) using ab96252 at 1/200 dilution in ICC/IF.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab96252 has been referenced in 7 publications.
- Tian JJ et al. Upregulation of Nav1.7 by endogenous hydrogen sulfide contributes to maintenance of neuropathic pain. Int J Mol Med 46:782-794 (2020). PubMed: 32468069
- Chen Y et al. O-GlcNAcylated c-Jun antagonizes ferroptosis via inhibiting GSH synthesis in liver cancer. Cell Signal 63:109384 (2019). PubMed: 31394193
- Song W et al. Edoxaban improves venous thrombosis via increasing hydrogen sulfide and homocysteine in rat model. Mol Med Rep 16:7706-7714 (2017). PubMed: 28944880
- Zhang R et al. Excessive nNOS/NO/AMPK signaling activation mediated by the blockage of the CBS/H2S system contributes to oxygen-glucose deprivation-induced endoplasmic reticulum stress in PC12 cells. Int J Mol Med 40:549-557 (2017). PubMed: 28656194
- Espe M et al. Methionine deficiency does not increase polyamine turnover through depletion of hepatic S-adenosylmethionine in juvenile Atlantic salmon. Br J Nutr 112:1274-85 (2014). PubMed: 25196630
- Kondo K et al. H2S protects against pressure overload-induced heart failure via upregulation of endothelial nitric oxide synthase. Circulation 127:1116-27 (2013). WB ; Mouse . PubMed: 23393010
- Kundu S et al. Hydrogen sulfide deficiency and diabetic renal remodeling: role of matrix metalloproteinase-9. Am J Physiol Endocrinol Metab 304:E1365-78 (2013). Mouse . PubMed: 23632630