- Datasheet
- Specific References (2)
- Protocols
Overview
-
Product nameAnti-CBS antibody
See all CBS primary antibodies -
DescriptionRabbit polyclonal to CBS
-
Host speciesRabbit
-
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
-
Species reactivityReacts with: Human, Zebrafish
Predicted to work with: Mouse, Rat, Rabbit, Cow
-
Immunogen
Recombinant fragment corresponding to a region within amino acids 39-221 of Human CBS (AAH00440).
-
Positive control
- Molt-4 and Raji whole cell lysates; OVCAR3 xenograft; 293T, H1299 and HeLa cell lysates.
Properties
-
FormLiquid
-
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
-
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol -
Concentration information loading... -
PurityImmunogen affinity purified
-
ClonalityPolyclonal
-
IsotypeIgG
-
Research areas
Associated products
-
Compatible Secondaries
-
Positive Controls
Applications
Our Abpromise guarantee covers the use of ab96252 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use a concentration of 5 µg/ml. | |
| WB | 1/500 - 1/3000. Predicted molecular weight: 61 kDa. | |
| IHC-P | 1/100 - 1/500. |
Target
-
FunctionOnly known pyridoxal phosphate-dependent enzyme that contains heme. Important regulator of hydrogen sulfide, especially in the brain, utilizing cysteine instead of serine to catalyze the formation of hydrogen sulfide. Hydrogen sulfide is a gastratransmitter with signaling and cytoprotective effects such as acting as a neuromodulator in the brain to protect neurons against hypoxic injury.
-
Tissue specificityIn the adult strongly expressed in liver and pancreas, some expression in heart and brain, weak expression in lung and kidney. In the fetus, expressed in brain, liver and kidney.
-
PathwayAmino-acid biosynthesis; L-cysteine biosynthesis; L-cysteine from L-homocysteine and L-serine: step 1/2.
-
Involvement in diseaseDefects in CBS are the cause of cystathionine beta-synthase deficiency (CBSD) [MIM:236200]. CBSD is an enzymatic deficiency resulting in altered sulfur metabolism and homocystinuria. The clinical features of untreated homocystinuria due to CBS deficiency include myopia, ectopia lentis, mental retardation, skeletal anomalies resembling Marfan syndrome, and thromboembolic events. Light skin and hair can also be present. Biochemical features include increased urinary homocystine and methionine.
-
Sequence similaritiesBelongs to the cysteine synthase/cystathionine beta-synthase family.
Contains 1 CBS domain. -
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
-
Database links
- Entrez Gene: 875 Human
- Entrez Gene: 12411 Mouse
- Entrez Gene: 24250 Rat
- Entrez Gene: 550524 Zebrafish
- Omim: 613381 Human
- SwissProt: P35520 Human
- SwissProt: Q91WT9 Mouse
- SwissProt: P32232 Rat
see all -
Alternative names
- AI047524 antibody
- AI303044 antibody
- Beta thionase antibody
see all
Images
-
Western blot - Anti-CBS antibody (ab96252)All lanes : Anti-CBS antibody (ab96252) at 1/5000 dilution
Lane 1 : Molt-4 whole cell lysate
Lane 2 : Raji whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 61 kDa
7.5% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CBS antibody (ab96252)Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft using ab96252 at 1/500 dilution. -
Immunocytochemistry/ Immunofluorescence - Anti-CBS antibody (ab96252)ICC/IF image of ab96252 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab96252, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. -
Western blot - Anti-CBS antibody (ab96252)All lanes : Anti-CBS antibody (ab96252) at 1 µg/ml
Lane 1 : Marker
Lane 2 : Zebrafish brain homogenate at 20 µg
Lane 3 : Zebrafish heart homogenate at 20 µg
Lane 4 : Zebrafish liver homogenate at 20 µg
Lane 5 : Human epithelial carcinoma cell line at 20 µg
Secondary
All lanes : Goat polyclonal to Rabbit IgG – H&L – Pre-Adsorbed (HRP) at 1/6000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 61 kDa
Observed band size: 72 kDa (why is the actual band size different from the predicted?)
Exposure time: 3 minutes
Protocols
References
This product has been referenced in:
- Kondo K et al. H2S protects against pressure overload-induced heart failure via upregulation of endothelial nitric oxide synthase. Circulation 127:1116-27 (2013). WB ; Mouse . Read more (PubMed: 23393010) »
- Kundu S et al. Hydrogen sulfide deficiency and diabetic renal remodeling: role of matrix metalloproteinase-9. Am J Physiol Endocrinol Metab 304:E1365-78 (2013). Mouse . Read more (PubMed: 23632630) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"