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Signal Transduction Metabolism Amino Acids
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-CBS antibody [EPR8578] (ab131155)

  • Datasheet
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Western blot - Anti-CBS antibody [EPR8578] (ab131155)
  • Western blot - Anti-CBS antibody [EPR8578] (ab131155)
  • OI-RD Scanning - Anti-CBS antibody [EPR8578] (ab131155)
  • Anti-CBS antibody [EPR8578] (ab131155)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8578] to CBS
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Human

You may also be interested in

Protein
Product image
Recombinant Human CBS protein (ab132564)
Knockout
Product image
Human CBS knockout HeLa cell lysate (ab257203)

View more associated products

Overview

  • Product name

    Anti-CBS antibody [EPR8578]
    See all CBS primary antibodies
  • Description

    Rabbit monoclonal [EPR8578] to CBS
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues near the N terminus of Human CBS (UniProt P35520).

  • Positive control

    • WB: Human fetal liver tissue lysate; HeLa and Raji cell lysates.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)

    KD = 6.78 x 10 -11 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR8578
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Metabolism
    • Amino Acids
    • Cancer
    • Cancer Metabolism
    • Cellular metabolic process
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Amino acid metabolism

Associated products

  • Alternative Versions

    • Anti-CBS antibody [EPR8578] - BSA and Azide free (ab248359)
  • Immunizing Peptide (Blocking)

    • CBS peptide (ab183541)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • KO cell lines

    • Human CBS knockout HeLa cell line (ab264950)
  • KO cell lysates

    • Human CBS knockout HeLa cell lysate (ab257203)
  • Recombinant Protein

    • Recombinant Human CBS protein (ab132564)

Applications

Our Abpromise guarantee covers the use of ab131155 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 61 kDa.Can be blocked with CBS peptide (ab183541).
  • Application notes
    Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • Target

    • Function

      Only known pyridoxal phosphate-dependent enzyme that contains heme. Important regulator of hydrogen sulfide, especially in the brain, utilizing cysteine instead of serine to catalyze the formation of hydrogen sulfide. Hydrogen sulfide is a gastratransmitter with signaling and cytoprotective effects such as acting as a neuromodulator in the brain to protect neurons against hypoxic injury.
    • Tissue specificity

      In the adult strongly expressed in liver and pancreas, some expression in heart and brain, weak expression in lung and kidney. In the fetus, expressed in brain, liver and kidney.
    • Pathway

      Amino-acid biosynthesis; L-cysteine biosynthesis; L-cysteine from L-homocysteine and L-serine: step 1/2.
    • Involvement in disease

      Defects in CBS are the cause of cystathionine beta-synthase deficiency (CBSD) [MIM:236200]. CBSD is an enzymatic deficiency resulting in altered sulfur metabolism and homocystinuria. The clinical features of untreated homocystinuria due to CBS deficiency include myopia, ectopia lentis, mental retardation, skeletal anomalies resembling Marfan syndrome, and thromboembolic events. Light skin and hair can also be present. Biochemical features include increased urinary homocystine and methionine.
    • Sequence similarities

      Belongs to the cysteine synthase/cystathionine beta-synthase family.
      Contains 1 CBS domain.
    • Cellular localization

      Cytoplasm. Nucleus.
    • Target information above from: UniProt accession P35520 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 875 Human
      • Omim: 613381 Human
      • SwissProt: P35520 Human
      • Unigene: 533013 Human
      • Alternative names

        • AI047524 antibody
        • AI303044 antibody
        • Beta thionase antibody
        • Beta-thionase antibody
        • Cbs antibody
        • Cbs cystathionine beta-synthase antibody
        • CBS_HUMAN antibody
        • Cystathionine beta synthase antibody
        • Cystathionine beta-synthase antibody
        • EC 4.2.1.22 antibody
        • HIP 4 antibody
        • HIP4 antibody
        • Methylcysteine synthase antibody
        • MGC18856 antibody
        • MGC18895 antibody
        • MGC37300 antibody
        • OTTHUMP00000109416 antibody
        • OTTHUMP00000109418 antibody
        • Serine sulfhydrase antibody
        see all

      Images

      • Western blot - Anti-CBS antibody [EPR8578] (ab131155)
        Western blot - Anti-CBS antibody [EPR8578] (ab131155)
        All lanes : Anti-CBS antibody [EPR8578] (ab131155) at 1/1000 dilution

        Lane 1 : Wild-type HeLa cell lysate
        Lane 2 : CBS knockout HeLa cell lysate
        Lane 3 : Raji cell lysate

        Lysates/proteins at 20 µg per lane.

        Performed under reducing conditions.

        Predicted band size: 61 kDa
        Observed band size: 70 kDa
        why is the actual band size different from the predicted?



        Lanes 1-3: Merged signal (red and green). Green - ab131155 observed at 70 kDa. Red - loading control, ab8245 observed at 37 kDa.

        ab131155 Anti-CBS antibody [EPR8578] was shown to specifically react with CBS in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264950 (knockout cell lysate ab257203) was used. Wild-type and CBS knockout samples were subjected to SDS-PAGE. ab131155 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

         

      • Western blot - Anti-CBS antibody [EPR8578] (ab131155)
        Western blot - Anti-CBS antibody [EPR8578] (ab131155)
        All lanes : Anti-CBS antibody [EPR8578] (ab131155) at 1/1000 dilution

        Lane 1 : Fetal liver lysate
        Lane 2 : Raji cell lysate

        Lysates/proteins at 10 µg per lane.

        Predicted band size: 61 kDa

      • OI-RD Scanning - Anti-CBS antibody [EPR8578] (ab131155)
        OI-RD Scanning - Anti-CBS antibody [EPR8578] (ab131155)
        Equilibrium disassociation constant (KD)
        Learn more about KD

        Click here to learn more about KD
      • Anti-CBS antibody [EPR8578] (ab131155)
        Anti-CBS antibody [EPR8578] (ab131155)

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (3)

      Publishing research using ab131155? Please let us know so that we can cite the reference in this datasheet.

      ab131155 has been referenced in 3 publications.

      • Lechuga TJ & Chen DB Analysis of Vascular Hydrogen Sulfide Biosynthesis. Methods Mol Biol 2007:19-36 (2019). PubMed: 31148104
      • You X  et al. Endogenous hydrogen sulfide contributes to uterine quiescence during pregnancy. Reproduction 153:535-543 (2017). PubMed: 28188160
      • Hu TX  et al. MiR 20a,-20b and -200c are involved in hydrogen sulfide stimulation of VEGF production in human placental trophoblasts. Placenta 39:101-10 (2016). PubMed: 26992682

      Customer reviews and Q&As

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