Recombinant Anti-CBS antibody [EPR8578] (ab131155)
![Western blot - Anti-CBS antibody [EPR8578] (ab131155)](https://www.abcam.com/ps/products/131/ab131155/Images/ab131155-384474-anti-cbs-antibody-epr8578-western-blot-hela-raji.jpg "Western blot - Anti-CBS antibody [EPR8578] (ab131155)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8578] to CBS
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-CBS antibody [EPR8578]
See all CBS primary antibodies -
Description
Rabbit monoclonal [EPR8578] to CBS -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC,IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to residues near the N terminus of Human CBS (UniProt P35520).
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Positive control
- WB: Human fetal liver tissue lysate; HeLa and Raji cell lysates.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 6.78 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR8578 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Immunizing Peptide (Blocking)
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Isotype control
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KO cell lines
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KO cell lysates
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab131155 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 61 kDa.Can be blocked with CBS peptide (ab183541). |
Target
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Function
Only known pyridoxal phosphate-dependent enzyme that contains heme. Important regulator of hydrogen sulfide, especially in the brain, utilizing cysteine instead of serine to catalyze the formation of hydrogen sulfide. Hydrogen sulfide is a gastratransmitter with signaling and cytoprotective effects such as acting as a neuromodulator in the brain to protect neurons against hypoxic injury. -
Tissue specificity
In the adult strongly expressed in liver and pancreas, some expression in heart and brain, weak expression in lung and kidney. In the fetus, expressed in brain, liver and kidney. -
Pathway
Amino-acid biosynthesis; L-cysteine biosynthesis; L-cysteine from L-homocysteine and L-serine: step 1/2. -
Involvement in disease
Defects in CBS are the cause of cystathionine beta-synthase deficiency (CBSD) [MIM:236200]. CBSD is an enzymatic deficiency resulting in altered sulfur metabolism and homocystinuria. The clinical features of untreated homocystinuria due to CBS deficiency include myopia, ectopia lentis, mental retardation, skeletal anomalies resembling Marfan syndrome, and thromboembolic events. Light skin and hair can also be present. Biochemical features include increased urinary homocystine and methionine. -
Sequence similarities
Belongs to the cysteine synthase/cystathionine beta-synthase family.
Contains 1 CBS domain. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 875 Human
- Omim: 613381 Human
- SwissProt: P35520 Human
- Unigene: 533013 Human
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Alternative names
- AI047524 antibody
- AI303044 antibody
- Beta thionase antibody
see all
Images
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Western blot - Anti-CBS antibody [EPR8578] (ab131155)All lanes : Anti-CBS antibody [EPR8578] (ab131155) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : CBS knockout HeLa cell lysate
Lane 3 : Raji cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 61 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?Lanes 1-3: Merged signal (red and green). Green - ab131155 observed at 70 kDa. Red - loading control, ab8245 observed at 37 kDa.
ab131155 Anti-CBS antibody [EPR8578] was shown to specifically react with CBS in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264950 (knockout cell lysate ab257203) was used. Wild-type and CBS knockout samples were subjected to SDS-PAGE. ab131155 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-CBS antibody [EPR8578] (ab131155)](https://www.abcam.com/ps/products/131/ab131155/Images/ab131155-145421-anti-cbs-antibody-epr8578-western-blot.jpg)
Western blot - Anti-CBS antibody [EPR8578] (ab131155)All lanes : Anti-CBS antibody [EPR8578] (ab131155) at 1/1000 dilution
Lane 1 : Fetal liver lysate
Lane 2 : Raji cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 61 kDa -
![OI-RD Scanning - Anti-CBS antibody [EPR8578] (ab131155)](https://www.abcam.com/ps/products/131/ab131155/Images/ab131155-145420-anti-cbs-antibody-epr8578.jpg)
OI-RD Scanning - Anti-CBS antibody [EPR8578] (ab131155) -
![Anti-CBS antibody [EPR8578] (ab131155)](https://www.abcam.com/ps/products/131/ab131155/Images/ab131155-4-benefits-of-recombinant-antibodies.png)
Anti-CBS antibody [EPR8578] (ab131155)
Datasheets and documents
References (3)
ab131155 has been referenced in 3 publications.
- Lechuga TJ & Chen DB Analysis of Vascular Hydrogen Sulfide Biosynthesis. Methods Mol Biol 2007:19-36 (2019). PubMed: 31148104
- You X et al. Endogenous hydrogen sulfide contributes to uterine quiescence during pregnancy. Reproduction 153:535-543 (2017). PubMed: 28188160
- Hu TX et al. MiR 20a,-20b and -200c are involved in hydrogen sulfide stimulation of VEGF production in human placental trophoblasts. Placenta 39:101-10 (2016). PubMed: 26992682
