Recombinant
RabMAb

Recombinant Anti-CBS antibody [EPR8579] (ab140600)

Overview

  • Product name

    Anti-CBS antibody [EPR8579]
    See all CBS primary antibodies
  • Description

    Rabbit monoclonal [EPR8579] to CBS
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human CBS aa 450-550. The exact sequence is proprietary.

  • Positive control

    • Recombinant Human CBS protein (ab132564) can be used as a positive control in WB. Human fetal liver, Raji, HeLa, and MCF7 cell lysates, Human pancreas tissue, MCF7 cells
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab140600 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 61 kDa.
IHC-P 1/100 - 1/250. Antigen retrieval is recommended.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function

      Only known pyridoxal phosphate-dependent enzyme that contains heme. Important regulator of hydrogen sulfide, especially in the brain, utilizing cysteine instead of serine to catalyze the formation of hydrogen sulfide. Hydrogen sulfide is a gastratransmitter with signaling and cytoprotective effects such as acting as a neuromodulator in the brain to protect neurons against hypoxic injury.
    • Tissue specificity

      In the adult strongly expressed in liver and pancreas, some expression in heart and brain, weak expression in lung and kidney. In the fetus, expressed in brain, liver and kidney.
    • Pathway

      Amino-acid biosynthesis; L-cysteine biosynthesis; L-cysteine from L-homocysteine and L-serine: step 1/2.
    • Involvement in disease

      Defects in CBS are the cause of cystathionine beta-synthase deficiency (CBSD) [MIM:236200]. CBSD is an enzymatic deficiency resulting in altered sulfur metabolism and homocystinuria. The clinical features of untreated homocystinuria due to CBS deficiency include myopia, ectopia lentis, mental retardation, skeletal anomalies resembling Marfan syndrome, and thromboembolic events. Light skin and hair can also be present. Biochemical features include increased urinary homocystine and methionine.
    • Sequence similarities

      Belongs to the cysteine synthase/cystathionine beta-synthase family.
      Contains 1 CBS domain.
    • Cellular localization

      Cytoplasm. Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • AI047524 antibody
      • AI303044 antibody
      • Beta thionase antibody
      • Beta-thionase antibody
      • Cbs antibody
      • Cbs cystathionine beta-synthase antibody
      • CBS_HUMAN antibody
      • Cystathionine beta synthase antibody
      • Cystathionine beta-synthase antibody
      • EC 4.2.1.22 antibody
      • HIP 4 antibody
      • HIP4 antibody
      • Methylcysteine synthase antibody
      • MGC18856 antibody
      • MGC18895 antibody
      • MGC37300 antibody
      • OTTHUMP00000109416 antibody
      • OTTHUMP00000109418 antibody
      • Serine sulfhydrase antibody
      see all

    Images

    • All lanes : Anti-CBS antibody [EPR8579] (ab140600) at 1/1000 dilution

      Lane 1 : Human fetal liver cell lysate
      Lane 2 : Raji cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : MCF7 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 61 kDa

    • Immunohistochemical analysis of paraffin embedded Human pancreas tissue labelling CBS with ab140600 antibody at a dilution of 1/100.
    • Immunofluorescent analysis of MCF7 cells labelling CBS with ab140600 at 1/100 dilution.

    References

    ab140600 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (breast (MCF7)and lung (A459) cancer cell line)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    25 µg
    Specification
    breast (MCF7)and lung (A459) cancer cell line
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C

    Abcam user community

    Verified customer

    Submitted Jun 30 2016

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