Anti-CBX1 / HP1 beta antibody [MAC353] (ab10811)
Key features and details
- Rat monoclonal [MAC353] to CBX1 / HP1 beta
- Suitable for: WB, ICC/IF, IP, ELISA, IHC-Fr, ChIP
- Knockout validated
- Reacts with: Mouse, Human
- Isotype: IgG2b
Overview
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Product name
Anti-CBX1 / HP1 beta antibody [MAC353]
See all CBX1 / HP1 beta primary antibodies -
Description
Rat monoclonal [MAC353] to CBX1 / HP1 beta -
Host species
Rat -
Specificity
Ab10811 recognises the M31 molecule in mouse and the homologous HP1 HS beta molecule in man. -
Tested applications
Suitable for: WB, ICC/IF, IP, ELISA, IHC-Fr, ChIPmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Fusion protein corresponding to Mouse CBX1/ HP1 beta (C terminal).
Database link: P83917 -
Positive control
- Murine and human nuclear extracts.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: 5% BSA -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
MAC353 -
Isotype
IgG2b -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab10811 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
Use at an assay dependent concentration. Detects a band of approximately 26 kDa (predicted molecular weight: 22.2 kDa).
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ICC/IF | (1) |
Use at an assay dependent concentration.
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IP |
Use at an assay dependent concentration.
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ELISA |
Use at an assay dependent concentration.
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IHC-Fr |
Use at an assay dependent concentration.
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ChIP |
Use at an assay dependent concentration.
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Notes |
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WB
Use at an assay dependent concentration. Detects a band of approximately 26 kDa (predicted molecular weight: 22.2 kDa). |
ICC/IF
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. |
ELISA
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
ChIP
Use at an assay dependent concentration. |
Target
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Function
Component of heterochromatin. Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. Interaction with lamin B receptor (LBR) can contribute to the association of the heterochromatin with the inner nuclear membrane. -
Tissue specificity
Expressed in all adult and embryonic tissues. -
Sequence similarities
Contains 2 chromo domains. -
Post-translational
modificationsNot phosphorylated.
Ubiquitinated. -
Cellular localization
Nucleus. Unassociated with chromosomes during mitosis. - Information by UniProt
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Database links
- Entrez Gene: 10951 Human
- Entrez Gene: 12412 Mouse
- Omim: 604511 Human
- SwissProt: P83916 Human
- SwissProt: P83917 Mouse
- Unigene: 77254 Human
- Unigene: 29055 Mouse
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Alternative names
- CBX 1 antibody
- CBX antibody
- Cbx1 antibody
see all
Images
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Lane 1: Wild-type HAP1 cell lysate (40 µg)
Lane 2: CBX1 knockout HAP1 cell lysate (40 µg)
Lane 3: MCF7 cell lysate (40 µg)
Lane 4: A431 cell lysate (40 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab10811 observed at 26 kDa. Red - loading control, ab181602, observed at 37 kDa.ab10811 was shown to specifically react with CBX1 / HP1 beta when CBX1 / HP1 beta knockout samples were used. Wild-type and CBX1 / HP1 beta knockout samples were subjected to SDS-PAGE. Ab10811 and ab181602 (loading control to GAPDH) were diluted at 1/500 and 1/10000 dilution respectively and incubated overnight at 4C. Blots were developed with Goat anti-Rat IgG H&L (IRDye® 800CW) (ab253031) preadsorbed and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777)secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
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HeLA cells were stained with ab10811 in panel one. In panel two they were stained with ab10811 (green), DAPI (blue) and SH-CREST (red), which stains the centromeres. Fix 30 minutes on ice in 4% formaldehyde in PEM. Quench autofluorescence 2 x 5 minutes with 1 mg/ml Na borohydride or 100 mM ammonium chloride in PEM. Permeablize 30 minutes with 0.5% TX-100 in PEM. Block 30 minutes in 5% milk in TBST. Primary antibody incubated overnight at 4oC diluted 1/100 in 5% milk in TBST. Secondary antibody incubated 1 hour at RT diluted in 5% milk in TBST. Post-fix 20 minutes on ice in 4% formaldehyde in PEM. Quench autofluorescence 2 x 5 minutes with ammonium chloride in PEM. Counterstain with DAPI in TBST. Mount with ProLong Gold antifade reagent from Invitrogen. Notes: Ample washing between each step. TBST = Tris buffered saline + 0.1% Tween. PEM = 80 mM K-PIPES, pH 6.8, 5 mM EGTA, 2 mM MgCl2.
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Immunofluorescence analysis of mouse erythroleukemia cell nuclei, staining CBX1 / HP1 beta in heterochromatin, with ab10811.
Cells were fixed with paraformaldehyde, permeabilized using Triton X-100 and blocked for 30 min with 2.5% BSA. Cells were incubated with primary antibody (1/50) before incubating with a Cy3-conjugated goat anti-rat IgG to detect staining.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (13)
ab10811 has been referenced in 13 publications.
- Lamb KN et al. Discovery of Potent Peptidomimetic Antagonists for Heterochromatin Protein 1 Family Proteins. ACS Omega 7:716-732 (2022). PubMed: 35036738
- Otake K et al. CENP-B creates alternative epigenetic chromatin states permissive for CENP-A or heterochromatin assembly. J Cell Sci 133:N/A (2020). PubMed: 32661090
- Yang G et al. The histone H3K9 methyltransferase SUV39H links SIRT1 repression to myocardial infarction. Nat Commun 8:14941 (2017). PubMed: 28361889
- Himeda CL et al. CRISPR/dCas9-mediated Transcriptional Inhibition Ameliorates the Epigenetic Dysregulation at D4Z4 and Represses DUX4-fl in FSH Muscular Dystrophy. Mol Ther 24:527-35 (2016). ChIP ; Human . PubMed: 26527377
- Liu J et al. HP1a mediates defective heterochromatin repair and accelerates senescence in Zmpste24-deficient cells. Cell Cycle 13:1237-47 (2014). PubMed: 24584199