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    cbx1-hp1-beta-antibody-ab152144.pdf

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Epigenetics and Nuclear Signaling Chromatin Binding Proteins Methyl Lysine
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Anti-CBX1 / HP1 beta antibody (ab152144)

  • Datasheet
  • SDS
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Western blot - Anti-CBX1 / HP1 beta  antibody (ab152144)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CBX1 / HP1 beta  antibody (ab152144)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CBX1 / HP1 beta  antibody (ab152144)

Key features and details

  • Rabbit polyclonal to CBX1 / HP1 beta
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Human
  • Isotype: IgG

You may also be interested in

Protein
Product image
Recombinant Human CBX1 / HP1 beta protein (ab109847)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-CBX1 / HP1 beta antibody
    See all CBX1 / HP1 beta primary antibodies
  • Description

    Rabbit polyclonal to CBX1 / HP1 beta
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Chicken, Xenopus laevis
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1-159 of Human CBX1/ HP1 beta.

  • Positive control

    • HeLa, HepG2 and HCT116 cell lysates; Human 59T xenograft and Mouse C2C12 xenograft tissues.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Chromatin Binding Proteins
    • Methyl Lysine

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab29545)
  • Recombinant Protein

    • Recombinant Human CBX1 / HP1 beta protein (ab109847)
  • Related Products

    • Recombinant Human CBX1 / HP1 beta protein (ab109847)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab152144 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/500 - 1/3000. Predicted molecular weight: 21 kDa.
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).
Notes
WB
1/500 - 1/3000. Predicted molecular weight: 21 kDa.
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).

Target

  • Function

    Component of heterochromatin. Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. Interaction with lamin B receptor (LBR) can contribute to the association of the heterochromatin with the inner nuclear membrane.
  • Tissue specificity

    Expressed in all adult and embryonic tissues.
  • Sequence similarities

    Contains 2 chromo domains.
  • Post-translational
    modifications

    Not phosphorylated.
    Ubiquitinated.
  • Cellular localization

    Nucleus. Unassociated with chromosomes during mitosis.
  • Target information above from: UniProt accession P83916 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 10951 Human
    • Entrez Gene: 12412 Mouse
    • Omim: 604511 Human
    • SwissProt: P83916 Human
    • SwissProt: P83917 Mouse
    • Unigene: 77254 Human
    • Unigene: 29055 Mouse
    • Alternative names

      • CBX 1 antibody
      • CBX antibody
      • Cbx1 antibody
      • CBX1_HUMAN antibody
      • Chromobox 1 antibody
      • Chromobox homolog 1 (HP1 beta homolog Drosophila ) antibody
      • chromobox homolog 1 antibody
      • Chromobox protein homolog 1 antibody
      • Drosophila HP1 beta antibody
      • Heterochromatin protein 1 beta antibody
      • Heterochromatin protein 1 homolog beta antibody
      • Heterochromatin protein p25 antibody
      • heterochromatin protein p25 beta antibody
      • HP1 beta antibody
      • HP1 beta homolog antibody
      • HP1 beta homolog Drosophila antibody
      • HP1, Drosophila. homolog of, beta antibody
      • HP1beta antibody
      • HP1Hs beta antibody
      • HP1Hsbeta antibody
      • Human heterochromatin protein p25 mRNA complete cds antibody
      • M31 antibody
      • MOD 1 antibody
      • MOD1 antibody
      • Modifier 1 protein antibody
      • p25beta antibody
      see all

    Images

    • Western blot - Anti-CBX1 / HP1 beta  antibody (ab152144)
      Western blot - Anti-CBX1 / HP1 beta antibody (ab152144)
      Anti-CBX1 / HP1 beta antibody (ab152144) at 1/1000 dilution + HeLa whole cell lysate at 30 µg

      Predicted band size: 21 kDa



      12% SDS PAGE
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CBX1 / HP1 beta  antibody (ab152144)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CBX1 / HP1 beta antibody (ab152144)
      Immunohistochemical analysis of paraffin-embedded 59T xenograft labeling CBX1 / HP1 beta with ab152144 at 1/500 dilution.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CBX1 / HP1 beta  antibody (ab152144)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CBX1 / HP1 beta antibody (ab152144)
      Immunohistochemical analysis of paraffin-embedded C2C12 xenograft labeling CBX1 / HP1 beta with ab152144 at 1/500 dilution.

    Protocols

    • Western blot protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab152144? Please let us know so that we can cite the reference in this datasheet.

    ab152144 has not yet been referenced specifically in any publications.

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