Anti-CBX1 / HP1 beta antibody (ab152144)
Key features and details
- Rabbit polyclonal to CBX1 / HP1 beta
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-CBX1 / HP1 beta antibody
See all CBX1 / HP1 beta primary antibodies -
Description
Rabbit polyclonal to CBX1 / HP1 beta -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Chicken, Xenopus laevis
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Immunogen
Recombinant fragment, corresponding to a region within amino acids 1-159 of Human CBX1/ HP1 beta.
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Positive control
- HeLa, HepG2 and HCT116 cell lysates; Human 59T xenograft and Mouse C2C12 xenograft tissues.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab152144 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 21 kDa.
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| IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).
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| Notes |
|---|
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WB
1/500 - 1/3000. Predicted molecular weight: 21 kDa. |
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IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0). |
Target
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Function
Component of heterochromatin. Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. Interaction with lamin B receptor (LBR) can contribute to the association of the heterochromatin with the inner nuclear membrane. -
Tissue specificity
Expressed in all adult and embryonic tissues. -
Sequence similarities
Contains 2 chromo domains. -
Post-translational
modificationsNot phosphorylated.
Ubiquitinated. -
Cellular localization
Nucleus. Unassociated with chromosomes during mitosis. - Information by UniProt
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Database links
- Entrez Gene: 10951 Human
- Entrez Gene: 12412 Mouse
- Omim: 604511 Human
- SwissProt: P83916 Human
- SwissProt: P83917 Mouse
- Unigene: 77254 Human
- Unigene: 29055 Mouse
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Alternative names
- CBX 1 antibody
- CBX antibody
- Cbx1 antibody
see all
Images
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Western blot - Anti-CBX1 / HP1 beta antibody (ab152144)Anti-CBX1 / HP1 beta antibody (ab152144) at 1/1000 dilution + HeLa whole cell lysate at 30 µg
Predicted band size: 21 kDa
12% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CBX1 / HP1 beta antibody (ab152144)Immunohistochemical analysis of paraffin-embedded 59T xenograft labeling CBX1 / HP1 beta with ab152144 at 1/500 dilution. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CBX1 / HP1 beta antibody (ab152144)Immunohistochemical analysis of paraffin-embedded C2C12 xenograft labeling CBX1 / HP1 beta with ab152144 at 1/500 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab152144 has not yet been referenced specifically in any publications.