Overview

  • Product name

    Anti-CBX1 / HP1 beta antibody
    See all CBX1 / HP1 beta primary antibodies
  • Description

    Rabbit polyclonal to CBX1 / HP1 beta
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Chicken, Xenopus laevis
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1-159 of Human CBX1/ HP1 beta.

  • Positive control

    • HeLa, HepG2 and HCT116 cell lysates; Human 59T xenograft and Mouse C2C12 xenograft tissues.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab152144 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 21 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).

Target

  • Function

    Component of heterochromatin. Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. Interaction with lamin B receptor (LBR) can contribute to the association of the heterochromatin with the inner nuclear membrane.
  • Tissue specificity

    Expressed in all adult and embryonic tissues.
  • Sequence similarities

    Contains 2 chromo domains.
  • Post-translational
    modifications

    Not phosphorylated.
    Ubiquitinated.
  • Cellular localization

    Nucleus. Unassociated with chromosomes during mitosis.
  • Information by UniProt
  • Database links

  • Alternative names

    • CBX 1 antibody
    • CBX antibody
    • Cbx1 antibody
    • CBX1_HUMAN antibody
    • Chromobox 1 antibody
    • Chromobox homolog 1 (HP1 beta homolog Drosophila ) antibody
    • chromobox homolog 1 antibody
    • Chromobox protein homolog 1 antibody
    • Drosophila HP1 beta antibody
    • Heterochromatin protein 1 beta antibody
    • Heterochromatin protein 1 homolog beta antibody
    • Heterochromatin protein p25 antibody
    • heterochromatin protein p25 beta antibody
    • HP1 beta antibody
    • HP1 beta homolog antibody
    • HP1 beta homolog Drosophila antibody
    • HP1, Drosophila. homolog of, beta antibody
    • HP1beta antibody
    • HP1Hs beta antibody
    • HP1Hsbeta antibody
    • Human heterochromatin protein p25 mRNA complete cds antibody
    • M31 antibody
    • MOD 1 antibody
    • MOD1 antibody
    • Modifier 1 protein antibody
    • p25beta antibody
    see all

Images

  • Anti-CBX1 / HP1 beta antibody (ab152144) at 1/1000 dilution + HeLa whole cell lysate at 30 µg

    Predicted band size: 21 kDa



    12% SDS PAGE
  • Immunohistochemical analysis of paraffin-embedded 59T xenograft labeling CBX1 / HP1 beta with ab152144 at 1/500 dilution.
  • Immunohistochemical analysis of paraffin-embedded C2C12 xenograft labeling CBX1 / HP1 beta with ab152144 at 1/500 dilution.

References

ab152144 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab152144.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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