Overview

  • Product name

  • Description

    Rabbit polyclonal to CBX4
  • Host species

    Rabbit
  • Specificity

    Two alternatively spliced transcript variants have been observed.
  • Tested applications

    Suitable for: ICC/IF, WB, ChIPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    A 17 amino acid synthetic peptide near the amino terminus of Human CBX4 (NP_003646).

  • Positive control

    • Human brain tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab139815 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 20 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 32, 61 kDa.
ChIP Use at an assay dependent concentration. PubMed: 25056311

Target

  • Function

    E3 SUMO-protein ligase which facilitates SUMO1 conjugation by UBE2I. Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility.
  • Tissue specificity

    Ubiquitous.
  • Pathway

    Protein modification; protein sumoylation.
  • Sequence similarities

    Contains 1 chromo domain.
  • Post-translational
    modifications

    Phosphorylated on Thr-497 by HIPK2 upon DNA damage; which enhances E3 SUMO-protein ligase activity and promotes sumoylation on Lys-494.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • CBX 4 antibody
    • CBX4 antibody
    • Cbx4 chromobox homolog 4 (Drosophila Pc class) antibody
    • CBX4_HUMAN antibody
    • Chromobox homolog 4 (Pc class homolog, Drosophila) antibody
    • Chromobox homolog 4 antibody
    • Chromobox protein homolog 4 antibody
    • E3 SUMO protein ligase CBX 4 antibody
    • E3 SUMO protein ligase CBX4 antibody
    • E3 SUMO-protein ligase CBX4 antibody
    • hPc 2 antibody
    • hPc2 antibody
    • NBP 16 antibody
    • NBP16 antibody
    • NS5ATP1 binding protein 16 antibody
    • Pc 2 antibody
    • Pc class 2 homolog antibody
    • Pc class homolog antibody
    • Pc class homolog Drosophila antibody
    • PC2 antibody
    • Polycomb 2 homolog antibody
    see all

Images

  • Immunofluorescence of CBX4 in Human brain tissue with ab139815 at 20 ug/mL.
  • All lanes : Anti-CBX4 antibody (ab139815) at 1 µg/ml

    Lane 1 : Human brain tissue lysate
    Lane 2 : Human brain tissue lysate with blocking peptide

    Lysates/proteins at 15 µg per lane.

    Predicted band size: 32, 61 kDa

References

This product has been referenced in:

  • Ren X  et al. Maintenance of Nucleolar Homeostasis by CBX4 Alleviates Senescence and Osteoarthritis. Cell Rep 26:3643-3656.e7 (2019). Read more (PubMed: 30917318) »
  • Pradeepa MM  et al. Psip1/Ledgf p75 restrains Hox gene expression by recruiting both trithorax and polycomb group proteins. Nucleic Acids Res 42:9021-32 (2014). ChIP . Read more (PubMed: 25056311) »
See all 2 Publications for this product

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