Recombinant
RabMAb

Anti-CBX4 antibody [EPR12288] (ab174300)

Overview

  • Product name
    Anti-CBX4 antibody [EPR12288]
    See all CBX4 primary antibodies
  • Description
    Rabbit monoclonal [EPR12288] to CBX4
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human CBX4 aa 500 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: O00257

  • Positive control
    • Molt4, Jurkat, 293T and Human thymus lysates; Jurkat cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab174300 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 61 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • Target

    • Function
      E3 SUMO-protein ligase which facilitates SUMO1 conjugation by UBE2I. Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility.
    • Tissue specificity
      Ubiquitous.
    • Pathway
      Protein modification; protein sumoylation.
    • Sequence similarities
      Contains 1 chromo domain.
    • Post-translational
      modifications
      Phosphorylated on Thr-497 by HIPK2 upon DNA damage; which enhances E3 SUMO-protein ligase activity and promotes sumoylation on Lys-494.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • CBX 4 antibody
      • CBX4 antibody
      • Cbx4 chromobox homolog 4 (Drosophila Pc class) antibody
      • CBX4_HUMAN antibody
      • Chromobox homolog 4 (Pc class homolog, Drosophila) antibody
      • Chromobox homolog 4 antibody
      • Chromobox protein homolog 4 antibody
      • E3 SUMO protein ligase CBX 4 antibody
      • E3 SUMO protein ligase CBX4 antibody
      • E3 SUMO-protein ligase CBX4 antibody
      • hPc 2 antibody
      • hPc2 antibody
      • NBP 16 antibody
      • NBP16 antibody
      • NS5ATP1 binding protein 16 antibody
      • Pc 2 antibody
      • Pc class 2 homolog antibody
      • Pc class homolog antibody
      • Pc class homolog Drosophila antibody
      • PC2 antibody
      • Polycomb 2 homolog antibody
      see all

    Images

    • All lanes : Anti-CBX4 antibody [EPR12288] (ab174300) at 1/10000 dilution

      Lane 1 : Molt4 lysate
      Lane 2 : Jurkat lysate
      Lane 3 : 293T lysate
      Lane 4 : Human thymus lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 61 kDa

    References

    ab174300 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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