Overview

  • Product name

  • Description

    Rabbit polyclonal to cbx7
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rabbit, Horse, Cow, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide within Human cbx7 aa 201-251. The exact sequence is proprietary.
    Database link: O95931

  • Positive control

    • Human Breast Carcinoma, Human Colon Carcinoma, Human Non-Small Cell Lung Cancer, Human Ovarian Carcinoma, Human Prostate Carcinoma tissues

Properties

Applications

Our Abpromise guarantee covers the use of ab123492 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Relevance

    Control of cell proliferation by Polycomb group proteins (PcG) is an important facet of cellular homeostasis and its disruption can promote tumorigenesis. CBX7 is a novel PcG protein which is found to control the growth of normal cells. CBX7 is a gene that encodes a Polycomb protein, as shown by sequence homology, its interaction with Ring1 and its localization to nuclear Polycomb bodies. CBX7 extends the lifespan of a wide range of normal human cells and immortalizes mouse fibroblasts by downregulating expression of the Ink4a/Arf locus. CBX7 does not inter-function or colocalize with Bmi1, and both can exert their actions independently of each other as shown by reverse genetics. CBX7 was highly expressed in three different prostate cancer cell lines and present at elevated levels in normal prostate. (Gil et al., 2004).
  • Cellular localization

    Nuclear
  • Database links

  • Alternative names

    • Cbx 7 antibody
    • CBX7 antibody
    • Chromobox homolog 7 antibody
    • Chromobox protein homolog 7 antibody

Images

  • ab123492 at 1/250 dilution staining cbx7 in Human prostate carcinoma by Immunohistochemistry Formalin-fixed, Paraffin-embedded tissue. Detection: DAB staining

References

ab123492 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

The immunogen is derived from the human CBX7 at the following UniProt entry URL:

http://www.uniprot.org/uniprot/O95931 .

Alignment of the immunogen with the sequence provided gave poor results.
Unfortunately, alignments of the immunogens of three of four of our other antibodies against this protein were worse. I am waiting for the result for the fourth, ab123492, which I will send when I receive it.

Please do not hesitate to contact us if you need any more advice or information.

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