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I am interested in possibly using your CBX7 antibody (ab21873) in zebrafish. I was interested in this CBX7 antibody, in particular, since it seems have a more wide range of predicted species reactivity than your other CBX7 antibodies. I noticed that the epitope resides within 200 of the C-terminus of Human CBX7. What human CBX7 protein is this as there are three present in ensemble? My assumption is that it is CBX7-001 as it is the only protein > 200 amino acids.
Or, I have included the zebrafish CBX7 protein sequence below? Could you make any inferences about reactivity based on the % similarity of your product with the ZF protein?
Lastly, if you think this antibody would not be ideal, do any of your other CBX7 antibodies have more % similarity with the ZF protein?
Asked on Jun 14 2012
Following up my previous message, the antibody ab123492 has not been tested for zebrafish reactivity and is unlikely to react, based on alignment of the immunogen with the zebrafish sequence you provided.
Please let us know if you have any other questions.
Answered on Jun 14 2012