Product nameAnti-cbx7 antibody
See all cbx7 primary antibodies
DescriptionRabbit polyclonal to cbx7
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Cow, Chimpanzee
- This antibody gave a positive signal in MEF1 whole cell lysate and HeLa nuclear extract.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab31765 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 28 kDa).|
RelevanceControl of cell proliferation by Polycomb group proteins (PcG) is an important facet of cellular homeostasis and its disruption can promote tumorigenesis. CBX7 is a novel PcG protein which is found to control the growth of normal cells. CBX7 is a gene that encodes a Polycomb protein, as shown by sequence homology, its interaction with Ring1 and its localization to nuclear Polycomb bodies. CBX7 extends the lifespan of a wide range of normal human cells and immortalizes mouse fibroblasts by downregulating expression of the Ink4a/Arf locus. CBX7 does not inter-function or colocalize with Bmi1, and both can exert their actions independently of each other as shown by reverse genetics. CBX7 was highly expressed in three different prostate cancer cell lines and present at elevated levels in normal prostate. (Gil et al., 2004).
- Cbx 7 antibody
- CBX7 antibody
- Chromobox homolog 7 antibody
- Chromobox protein homolog 7 antibody
All lanes : Anti-cbx7 antibody (ab31765) at 1 µg/ml
Lane 1 : Hela nuclear Lysate
Lane 2 : Hela nuclear Lysate with Human cbx7 peptide (ab22423) at 1 µg/ml
Lysates/proteins at 20 µg per lane.
All lanes : Secondary ab: Alexa Fluor Goat polyclonal to Rabbit IgG (700)
Predicted band size: 28 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?
A clear band at the expected size appears in lane 1. This band is specifically blocked by the immunizing peptide in lane 2.
Anti-cbx7 antibody (ab31765) at 1 µg/ml + MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 28 kDa
Observed band size: 28 kDa
Additional bands at: 100 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
This product has been referenced in:
- Maertens GN et al. Ubiquitin-specific proteases 7 and 11 modulate Polycomb regulation of the INK4a tumour suppressor. EMBO J 29:2553-65 (2010). WB ; Human . Read more (PubMed: 20601937) »
- Mansueto G et al. Identification of a New Pathway for Tumor Progression: MicroRNA-181b Up-Regulation and CBX7 Down-Regulation by HMGA1 Protein. Genes Cancer 1:210-24 (2010). Read more (PubMed: 21779448) »