- Datasheet
- Specific References (25)
- Protocols
Overview
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Product nameAnti-cbx7 antibody - ChIP Grade
See all cbx7 primary antibodies -
DescriptionRabbit polyclonal to cbx7 - ChIP Grade
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Host speciesRabbit
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Tested applicationsSuitable for: ICC/IF, ChIP, WB, IHC-Pmore details
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Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Cow, Chimpanzee
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Immunogen
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Positive control
- ab21873 gave a positive result in the following nuclear lysates: Hela, Jurkat, HepG2. This antibody gave a positive result in IHC in the following FFPE tissue: Human normal protate.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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ChIP Related Products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab21873 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use at an assay dependent concentration. | |
| ChIP | Use at an assay dependent concentration. | |
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 28 kDa). | |
| IHC-P | Use a concentration of 1 µg/ml. |
Target
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RelevanceControl of cell proliferation by Polycomb group proteins (PcG) is an important facet of cellular homeostasis and its disruption can promote tumorigenesis. CBX7 is a novel PcG protein which is found to control the growth of normal cells. CBX7 is a gene that encodes a Polycomb protein, as shown by sequence homology, its interaction with Ring1 and its localization to nuclear Polycomb bodies. CBX7 extends the lifespan of a wide range of normal human cells and immortalizes mouse fibroblasts by downregulating expression of the Ink4a/Arf locus. CBX7 does not inter-function or colocalize with Bmi1, and both can exert their actions independently of each other as shown by reverse genetics. CBX7 was highly expressed in three different prostate cancer cell lines and present at elevated levels in normal prostate. (Gil et al., 2004).
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Cellular localizationNuclear
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Database links
- Entrez Gene: 525770 Cow
- Entrez Gene: 23492 Human
- Entrez Gene: 52609 Mouse
- Entrez Gene: 362962 Rat
- Omim: 608457 Human
- SwissProt: O95931 Human
- SwissProt: Q8VDS3 Mouse
- SwissProt: P60889 Rat
see all -
Alternative names
- Cbx 7 antibody
- CBX7 antibody
- Chromobox homolog 7 antibody
- Chromobox protein homolog 7 antibody
Images
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ChIP - Anti-cbx7 antibody - ChIP Grade (ab21873)Image courtesy of an anonymous AbReviewab21873 was used at 1/100 dilution in cross-linking ChIP analysis of mouse mES E14 cells. Positive control: cbx7 overexpressing cells. Negative control: RA-treated cells.
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Western blot - Anti-cbx7 antibody - ChIP Grade (ab21873)All lanes : Anti-cbx7 antibody - ChIP Grade (ab21873) at 1 µg
Lane 1 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
Lane 2 :Jurkat nuclear extract lysate (ab14844)
Lane 3 :HepG2 nuclear extract lysate (ab14660)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 28 kDa
Observed band size: 34 kDa (why is the actual band size different from the predicted?)
Additional bands at: 100 kDa, 70 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds -
Immunocytochemistry/ Immunofluorescence - Anti-cbx7 antibody - ChIP Grade (ab21873)Image courtesy of an Abreview submitted by Dr. Kirk McManus, Univ. of Manitoba/Cancer Care MICB, Canadaab21873 (1/1000) staining cbx7 in HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.5% Triton X-100/PBS and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please refer to Abreview. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-cbx7 antibody - ChIP Grade (ab21873)IHC image of cbx7 staining in Human normal prostate formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab21873, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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Western blot - Anti-cbx7 antibody - ChIP Grade (ab21873)All lanes : Anti-cbx7 antibody - ChIP Grade (ab21873) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 4 :Jurkat nuclear extract lysate (ab14844)
Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 6 :HepG2 nuclear extract lysate (ab14660)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Polyclonal to rabbit IgG - H&L - Pre adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 28 kDa
Observed band size: 34 kDa (why is the actual band size different from the predicted?)
Additional bands at: 100 kDa, 70 kDa. We are unsure as to the identity of these extra bands.
Protocols
References
This product has been referenced in:
- Tatavosian R et al. Live-cell single-molecule dynamics of PcG proteins imposed by the DIPG H3.3K27M mutation. Nat Commun 9:2080 (2018). Read more (PubMed: 29802243) »
- Takeshita S et al. Expression of P-REX2a is associated with poor prognosis in endometrial malignancies. Oncotarget 9:24778-24786 (2018). Read more (PubMed: 29872505) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"