Product nameAnti-Cbx8 antibody
See all Cbx8 primary antibodies
DescriptionRabbit polyclonal to Cbx8
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Xenopus tropicalis
Synthetic peptide corresponding to a region between residue 350 and the C-terminus (residue 389) of human Cbx8 (NP_065700.1).
- HeLa and 293T whole cell lysates.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab70796 was affinity purified using an epitope specific to Cbx8 immobilized on solid support.
Our Abpromise guarantee covers the use of ab70796 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/2000 - 1/10000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionComponent of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility.
Sequence similaritiesContains 1 chromo domain.
- Information by UniProt
- Cbx8 antibody
- CBX8_HUMAN antibody
- Chromobox Homolog 8 (Drosophila Pc Class) antibody
All lanes : Anti-Cbx8 antibody (ab70796) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 43 kDa
Observed band size: 43 kDa
Additional bands at: 28 kDa, 55 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes
Detection of Cbx8 by Western Blot of Immunoprecipitate.
ab70796 at 1µg/ml staining Cbx8 in HeLa whole cell lysate immunoprecipitated using ab70796 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 3 minutes.