Recombinant Anti-Cbx8 antibody [EPR23800-14] (ab259849)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23800-14] to Cbx8
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Cbx8 antibody [EPR23800-14]
See all Cbx8 primary antibodies -
Description
Rabbit monoclonal [EPR23800-14] to Cbx8 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: ChIP,Flow Cyt (Intra),ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa; 293T; Huh7; NIH/3T3 and C6 whole cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23800-14 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab259849 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Predicted molecular weight: 43 kDa.
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Notes |
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WB
1/1000. Predicted molecular weight: 43 kDa. |
Target
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Function
Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. -
Sequence similarities
Contains 1 chromo domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 57332 Human
- Entrez Gene: 30951 Mouse
- Entrez Gene: 303731 Rat
- SwissProt: Q9HC52 Human
- SwissProt: Q9QXV1 Mouse
- Unigene: 387258 Human
- Unigene: 99953 Mouse
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Alternative names
- Cbx8 antibody
- CBX8_HUMAN antibody
- Chromobox Homolog 8 (Drosophila Pc Class) antibody
see all
Images
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All lanes : Anti-Cbx8 antibody [EPR23800-14] (ab259849) at 1/1000 dilution
Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : 293T (human embryonic kidney epithelial cell) whole cell lysate
Lane 3 : Huh7 (human hepatocellar carcinoma epithelial cell) whole cell lysate
Lane 4 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
Lane 5 : C6 (rat glial tumor glial cell) whole cell lysate
Lysates/proteins at 40 µg per lane.
Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution (VeriBlot for IP secondary antibody(HRP))
Predicted band size: 43 kDa
Observed band size: 43,48 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
Incubation for primary antibody was carried out overnight at 4 degree.
This blot was developed using a higher sensitivity ECL substrate.
Exposure time: 48 seconds
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (1)
ab259849 has been referenced in 1 publication.
- Li J et al. Expression profile and prognostic values of Chromobox family members in human glioblastoma. Aging (Albany NY) 14:1910-1931 (2022). PubMed: 35210369