Key features and details
- Rabbit polyclonal to CC2D1A
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-CC2D1A antibody
See all CC2D1A primary antibodies
DescriptionRabbit polyclonal to CC2D1A
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Chimpanzee, Gorilla, Orangutan
Synthetic peptide corresponding to a region between residues 1 and 50 of human CC2D1A
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab70375 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 130 kDa (predicted molecular weight: 104 kDa).|
|IP||Use at 3-10 µg/mg of lysate.|
|IHC-P||1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
RelevanceCC2D1A belongs to the CC2D1 family. It contains one C2 domain. It is a transcription factor that binds specifically to the FRE (five repressor element) and represses HTR1A gene transcription in neuronal cells. The combination of calcium and ATP specifically inactivates the binding with FRE. CC2D1A may play a role in the altered regulation of HTR1A associated with anxiety and major depression. It also mediates HDAC-independent repression of HTR1A promoter in neuronal cell. There are three named isoforms. Defects in CC2D1A are the cause of non-syndromic mental retardation autosomal recessive type 3 (MRT3). Patients display severe mental retardation and psychomotor development delay in early childhood. Non-syndromic mental retardation patients do not manifest other clinical signs.
Cellular localizationCytoplasm. Nucleus.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue labelling CC2D1A with ab70375 at 1/1000 (1µg/ml). Detection: DAB.
Anti-CC2D1A antibody (ab70375) at 0.3 µg/ml + Whole cell lysate from 293T cells at 50 µg
Predicted band size: 104 kDa
Observed band size: 130 kDa why is the actual band size different from the predicted?
Additional bands at: 105 kDa, 120 kDa, 90 kDa. We are unsure as to the identity of these extra bands.
Detection of Human CC2D1A by Immunoprecipitiaton. ab70375 used at 20 µg/50 mg NETN or RIPA lysate from HeLa cells. Immunoprecipitate was resolved by SDS-PAGE and the band that is CC2D1A was confirmed by mass spectrometry.
ab70375 has not yet been referenced specifically in any publications.