Overview

  • Product name
    Anti-CCDC109B antibody - C-terminal
    See all CCDC109B primary antibodies
  • Description
    Rabbit polyclonal to CCDC109B - C-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human CCDC109B aa 298-328 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q9NWR8

  • Positive control
    • A549 cell lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab170715 is purified through a protein A column, followed by peptide affinity purification
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab170715 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 39 kDa.
IHC-P Use at an assay dependent concentration. PubMed: 28754121

Target

Images

  • Anti-CCDC109B antibody - C-terminal (ab170715) at 1/100 dilution + A549 cell lysate at 35 µg

    Predicted band size: 39 kDa

References

This product has been referenced in:
  • Xu R  et al. Coiled-coil domain containing 109B is a HIF1a-regulated gene critical for progression of human gliomas. J Transl Med 15:165 (2017). IHC-P ; Human . Read more (PubMed: 28754121) »
See 1 Publication for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - other (Fibroblast and brain tissue)
Gel Running Conditions
Reduced Denaturing (4-20% gradient)
Loading amount
100 µg
Specification
Fibroblast and brain tissue
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Sep 19 2016

Application
Western blot
Sample
Rat Cell lysate - whole cell (H9C2)
Gel Running Conditions
Non-reduced Denaturing
Loading amount
20 µg
Treatment
siRNA
Specification
H9C2
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Dec 11 2015

Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293T)
Gel Running Conditions
Reduced Denaturing (10% gel)
Loading amount
30 µg
Specification
HEK293T
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jul 01 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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