Key features and details
- Rabbit polyclonal to CCDC112
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-CCDC112 antibody
DescriptionRabbit polyclonal to CCDC112
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human CCDC112 aa 201-446.
Database link: Q8NEF3
- WB: Jurkat, HeLa, HepG2 and A549 cell lysate. IHC-P: Human kidney and rectum tissue. ICC/IF: MCF7 cells.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%
Our Abpromise guarantee covers the use of ab237742 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/200.|
|WB||1/500 - 1/2000.|
|ICC/IF||1/50 - 1/200.|
- Information by UniProt
- MBC1 antibody
- CC112_HUMAN antibody
- CCDC112 antibody
All lanes : Anti-CCDC112 antibody (ab237742) at 1/500 dilution
Lane 1 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 3 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 4 : A549 (Human lung carcinoma cell line) whole cell lysate
All lanes : Goat polyclonal to IgG at 1/10000 dilution
Paraffin-embedded human kidney tissue stained for CCDC112 using ab237742 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human rectum tissue stained for CCDC112 using ab237742 at 1/100 dilution in immunohistochemical analysis.
MCF7 (Human breast adenocarcinoma cell line) cells stained for CCDC112 (green) using ab237742 at 1/100 dilution in ICC/IF, followed by an Alexa-Fluor®488-conjugated goat anti-rabbit IgG (H+L).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab237742 has not yet been referenced specifically in any publications.