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Rabbit polyclonal to CCDC114
antigen sequence: SKDDQHLLQE QQQKVLQQRM DKVHSEAERL EARFQDVRGQ LEKLKADIQL LFTKAHCDSS MIDDLLGVKT SMGDRDMGLF LSLIEKRLVE, corresponding to amino acids of Human CCDC114.
- Human rectum tissue; RT 4, U 251 MG and Human tonsil lysates.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
Information by UniProt
- CC114_HUMAN antibody
- CCDC114 antibody
- CILD20 antibody
- Coiled-coil domain-containing protein 114 antibody
Immunocytochemistry/ Immunofluorescence - Anti-CCDC114 antibody (ab122798)
Immunofluorescent staining of Human cell line U-251 MG shows positivity in nucleoli and cytoplasm. Recommended concentration of ab122798 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Western blot - Anti-CCDC114 antibody (ab122798)
All lanes : Anti-CCDC114 antibody (ab122798) at 1/250 dilution
Lane 1 : RT 4 lysate
Lane 2 : U 251 MG lysate
Lane 3 : Human plasma lysate
Lane 4 : Human liver lysate
Lane 5 : Human tonsil lysate
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCDC114 antibody (ab122798)
ab122798, at 1/500 dilution, staining CCDC114 in Paraffin-embedded Human rectum tissue by
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"