Key features and details
- Rabbit polyclonal to CCDC124
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-CCDC124 antibody
See all CCDC124 primary antibodies
DescriptionRabbit polyclonal to CCDC124
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Dog, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide, corresponding to a region between residues 173 and 223 of Human CCDC124 (NP_612451.1)
- HeLa whole cell lysate.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab86079 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 26 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
Sequence similaritiesBelongs to the CCDC124 family.
- Information by UniProt
- 1810023B24Rik antibody
- CC124_HUMAN antibody
- CCDC124 antibody
All lanes : Anti-CCDC124 antibody (ab86079) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Predicted band size: 26 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?
Detection: chemiluminescence with exposure time of 1 minute
1mg whole cell lysate from HeLa cells was immunoprecipitated using 3µg ab86079.
20% of IP was loaded per lane, and probed with ab86079 at 1µg/ml (lane 1) or with a control IgG (lane 2). Detection: chemiluminescence with an exposure time of 10 seconds.
ab86079 has not yet been referenced specifically in any publications.