Overview

  • Product name

    Anti-CCDC170 antibody
  • Description

    Rabbit polyclonal to CCDC170
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human CCDC170 aa 442-701.
    Database link: NP_079335

  • Positive control

    • A549 whole cell lysate; HeLa, HepG2 and HCT116 cell lysates
  • General notes

     This product was previously labelled as C6orf97

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab97814 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/200.
WB 1/500 - 1/3000. Predicted molecular weight: 82 kDa.

Target

Images

  • Anti-CCDC170 antibody (ab97814) at 1/1000 dilution + A549 whole cells lysate at 30 µg

    Predicted band size: 82 kDa



    7.5% SDS Page
  • ab14404 staining CCDC170 - in Human Breast cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with Paraformaldehyde, permeabilized with Triton X-100 0.1% and blocked with 10% serum for 60 minutes at 21°C. Samples were incubated with primary antibody (1/200 in PBS + 10% FBS) for 1 hour at 21°C. A diluted(1/2500) Alexa Fluor®488-conjugated Goat anti-rabbit polyclonal was used as the secondary antibody.

    See Abreview

References

This product has been referenced in:

  • Jiang P  et al. The Protein Encoded by the CCDC170 Breast Cancer Gene Functions to Organize the Golgi-Microtubule Network. EBioMedicine 22:28-43 (2017). Read more (PubMed: 28687497) »
See 1 Publication for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (breast)
Specification
breast
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1 % triton X-100
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Sep 28 2012

Answer



I am very pleased to hear your customer would like to test ab97814 Anti-C6orf97 antibody in ICC/ IF. This code will give them 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview forICC/ IF and include this code in the "Additional Comments" section so we know the Abreview is for this promotion.Please remember that submission of the Abreview is sufficient for the discount code to become active.
For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback thatyour customercan provide will be greatly appreciated, whether positive or negative. If they have any further questions, please do not hesitate to contact us.


The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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