Product nameAnti-CCDC21 antibody
See all CCDC21 primary antibodies
DescriptionRabbit polyclonal to CCDC21
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide, corresponding to a region within amino acids 675-725 of Human CCDC21 (NP_073615.2).
- Jurkat, HeLa and 293T whole cell lysates.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab117782 was affinity purified using an epitope specific to CCDC21 immobilized on solid support.
Our Abpromise guarantee covers the use of ab117782 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 86 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
Sequence similaritiesBelongs to the CEP85 family.
Cellular localizationCytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > spindle pole. Nucleus > nucleolus. Localizes to centrosomes and nucleolus in interphase. Upon entry into mitosis, relocates from nucleolus and accumulates at spindle poles.
- Information by UniProt
- CCDC21 antibody
- Centrosomal protein of 85 kDa antibody
- CEP85 antibody
All lanes : Anti-CCDC21 antibody (ab117782) at 0.1 µg/ml
Lane 1 : 293T whole cell lysates at 50 µg
Lane 2 : 293T whole cell lysates at 15 µg
Lane 3 : Jurkat whole cell lysates at 50 µg
Lane 4 : HeLa whole cell lysates at 50 µg
Developed using the ECL technique.
Predicted band size: 86 kDa
Exposure time: 3 minutes
Detection of CCDC21 by Western Blot of Immunprecipitate.
ab117782 at 1µg/ml staining CCDC21 in 293T whole cell lysate immunoprecipitated using ab117782 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 30 seconds.
ab117782 has not yet been referenced specifically in any publications.