Product nameAnti-CCDC34/L15 antibody
DescriptionRabbit polyclonal to CCDC34/L15
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Human
- IHC-P: Human cerebral cortex tissue WB: RT4 and U251 MG cell lysates.
This product was previously labelled as CCDC34
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab122396 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
|WB||1/250 - 1/500.|
|IHC-P||1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
Involvement in diseaseNote=A chromosomal aberration involving CCDC34 is found in a patient with hamartoma of the retinal pigment epithelium and retina. Translocation t(11;18) (p13;p11.2).
- Information by UniProt
- CCD34_HUMAN antibody
- Ccdc34 antibody
- Coiled-coil domain-containing protein 34 antibody
All lanes : Anti-CCDC34/L15 antibody (ab122396) at 1/250 dilution
Lane 1 : RT4 cell lysate
Lane 2 : U251 MG cell lysate
Lane 3 : Human Plasma cell lysate
Lane 4 : Liver cell lysate
Lane 5 : Tonsil cell lysate
Immunofluorescent staining of Human cell line U-251 MG shows positivity in nucleus, nucleoli and nuclear membrane. Recommended concentration of ab122396 1-4 µg/ml. Cells treated with PFA/Triton X-100.
ab122430, at 1/500, staining CCDC34/L15 in paraffin embedded Human cerebral cortex tissue by immunohistochemistry.
This product has been referenced in:
- Hu DD et al. Overexpression of Coiled-Coil Domain-Containing Protein 34 (CCDC34) and its Correlation with Angiogenesis in Esophageal Squamous Cell Carcinoma. Med Sci Monit 24:698-705 (2018). Read more (PubMed: 29397026) »
- Liu LB et al. High Expression of CCDC34 Is Associated with Poor Survival in Cervical Cancer Patients. Med Sci Monit 24:8383-8390 (2018). Read more (PubMed: 30458457) »