Product nameAnti-CCDC47 antibody
See all CCDC47 primary antibodies
DescriptionRabbit polyclonal to CCDC47
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Cow, Xenopus laevis, Zebrafish, Orangutan
Synthetic peptide within Human CCDC47 aa 433-483. The exact sequence is proprietary.
Database link: Q96A33
- WB: HeLa, HEK-293T, Jurkat, TCMK-1 and NIH/3T3 whole cell lysate. IP: CCDC47 IP in HEK-293T whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntibody was affinity purified using an epitope specific to CCDC47 immobilized on solid support.
Our Abpromise guarantee covers the use of ab241608 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 56 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
- Information by UniProt
- CCD47_HUMAN antibody
- CCDC 47 antibody
- CCDC47 antibody
All lanes : Anti-CCDC47 antibody (ab241608) at 0.1 µg/ml
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-239T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : TCMK-1 (Mouse kidney epithelial cell line) whole cell lysate
Lane 5 : NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 56 kDa
Exposure time: 10 seconds
Prepared using NETN lysis buffer.
CCDC47 was immunoprecipitated from HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer.
ab241608 used for IP at 6 µg per reaction. For WB 0.4 µg/ml.
Lane 1: ab241608 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Chemiluminescence detection: 10 seconds.
ab241608 has not yet been referenced specifically in any publications.