Key features and details
- Rabbit polyclonal to CCDC50
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-CCDC50 antibody
DescriptionRabbit polyclonal to CCDC50
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Recombinant fragment, corresponding to a region within amino acids 1 -147 of Human CCDC50 (Q8IVM0).
- Lysates of: 293T, A431, HeLa Hepatoma
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We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab127169 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 36 kDa.|
|IHC-P||1/100 - 1/1000.|
|ICC/IF||1/100 - 1/1000.|
FunctionInvolved in EGFR signaling.
Tissue specificityIsoform 1 and isoform 2 are co-expressed in placenta, liver, lung, kidney and pancreas. Only isoform 1 is detected in skeletal muscle, brain and heart.
Involvement in diseaseDefects in CCDC50 are the cause of deafness autosomal dominant type 44 (DFNA44) [MIM:607453]. A form of non-syndromic hearing loss. It is initially moderate and affects mainly low to mid frequencies. Later, it progresses to involve all the frequencies and leads to a profound hearing loss by the 6th decade. The onset of the hearing loss occurs in the first decade of life.
modificationsPhosphorylated on tyrosine residues.
Cellular localizationCytoplasm. Associated with microtubules of the cytoskeleton and mitotic apparatus.
- Information by UniProt
- C3orf6 antibody
- C3orf6 YMER antibody
- CCD50_HUMAN antibody
ab127169, at 1/500 dilution, analysis of paraffin-embedded Hepatoma by immunohistochemical
All lanes : Anti-CCDC50 antibody (ab127169) at 1/5000 dilution
Lane 1 : 293T cell lysate
Lane 2 : A431 cell lysate
Lane 3 : HeLa cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 36 kDa
10% SDS PAGE
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using ab127169 (Green) at 1/500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1/2000.
ab127169 has been referenced in 1 publication.
- Sun G et al. HnRNP A1 - mediated alternative splicing of CCDC50 contributes to cancer progression of clear cell renal cell carcinoma via ZNF395. J Exp Clin Cancer Res 39:116 (2020). PubMed: 32560659