Key features and details
- Rabbit polyclonal to CCDC52
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-CCDC52 antibody
DescriptionRabbit polyclonal to CCDC52
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Rabbit, Horse, Cow, Dog, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
Synthetic peptide, corresponding to a region between amino acids 805-855 of Human CCDC52 (NP_653319.1).
- HeLa and 293T whole cell lysates.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab117801 was affinity purified using an epitope specific to CCDC52 immobilized on solid support.
Our Abpromise guarantee covers the use of ab117801 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 96 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionRegulator required for centriole duplication, for proper bipolar spindle formation and chromosome congression in mitosis.
Cellular localizationCytoplasm > cytoskeleton > centrosome > centriole. Cytoplasm > cytoskeleton > spindle.
- Information by UniProt
- Coiled coil domain containing 52 antibody
- Coiled coil domain containing protein 52 antibody
- Coiled-coil domain-containing protein 52 antibody
All lanes : Anti-CCDC52 antibody (ab117801) at 0.1 µg/ml
Lane 1 : 293T whole cell lysate at 50 µg
Lane 2 : 293T whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 96 kDa
Exposure time: 3 minutes
Lane 1: ab1177801 at 1 µg/ml staining CCDC52 in 293T cell lysate immunoprecipitated using ab117801 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Lane 2: control IgG. Predicted band size : 96 kDa. Exposure time: 10 seconds
ab117801 has not yet been referenced specifically in any publications.