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Our Abpromise guarantee covers the use of ab170357 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/100 - 1/500. Predicted molecular weight: 38 kDa.|
|Flow Cyt||1/10 - 1/50.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
Flow cytometry analysis of NCI-H460 cells labeling CCDC54 (green, right histogram), using ab170357 at a 1/10 dilution, and negative control cells (blue, left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies was used for the analysis.
Immunohistochemical analysis of formalin-fixed paraffin-embedded Human uterus tissue, labeling CCDC54 using ab170357 at a 1/50 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.
ab170357 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"