Overview

  • Product name
  • Description
    Rabbit polyclonal to CCDC69
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human CCDC69 aa 148-178 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary. (NP_056436.2)
    Database link: A6NI79

  • Positive control
    • NCI-H460 and mouse stomach cell lysates; NCI-H460 cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab175301 is purified through a protein A column, followed by peptide affinity purification.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab175301 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 35 kDa.
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

Images

  • Anti-CCDC69 antibody (ab175301) at 1/1000 dilution + NCI-H460 cell lysate at 35 µg

    Predicted band size: 35 kDa

  • Anti-CCDC69 antibody (ab175301) at 1/1000 dilution + mouse stomach cell lysate at 35 µg

    Predicted band size: 35 kDa

  • Flow cytometric analysis of NCI-H460 cells labeling CCDC69 (green, right histogram) using ab175301 at a 1/10 dilution, and negative control cells (blue, left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

References

This product has been referenced in:
  • Cui L  et al. Inhibition of coiled coil domain containing protein 69 enhances platinum-induced apoptosis in ovarian cancer cells. Oncotarget 8:101634-101648 (2017). Read more (PubMed: 29254192) »
See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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