Anti-CCDC76 antibody (ab186137)
Key features and details
- Rabbit polyclonal to CCDC76
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-CCDC76 antibody -
Description
Rabbit polyclonal to CCDC76 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Cat, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Xenopus tropicalis -
Immunogen
Synthetic peptide within Human CCDC76 aa 25-75. The exact sequence is proprietary. NP_061956.2.
Sequence:KKKRFCRMVVAAGKRFCGEHAGAAEE EDARKRILCPLDPKHTVYEDQLAKH
Database link: Q9NUP7 -
Positive control
- 293T, HeLa, Jurkat, TCMK and NIH 3T3 whole cell lysates.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7-8 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab186137 was affinity purified using an epitope specific to CCDC76 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab186137 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | 1/2000 - 1/10000. Predicted molecular weight: 52 kDa. |
Target
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Function
Specifically methylates guanosine-4 in various tRNAs with a Gly(CCG), His or Pro signature. -
Sequence similarities
Belongs to the TRM13 family.
Contains 1 CHHC-type zinc finger. - Information by UniProt
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Database links
- Entrez Gene: 101086829 Cat
- Entrez Gene: 742372 Chimpanzee
- Entrez Gene: 100730968 Guinea pig
- Entrez Gene: 100050460 Horse
- Entrez Gene: 54482 Human
- Entrez Gene: 229780 Mouse
- Entrez Gene: 102167932 Pig
- Entrez Gene: 100347251 Rabbit
see all -
Alternative names
- Ccdc76 antibody
- Coiled coil domain containing 76 antibody
- Coiled-coil domain-containing protein 76 antibody
see all
Images
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All lanes : Anti-CCDC76 antibody (ab186137) at 0.1 µg/ml
Lane 1 : 293T cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : TCMK cell lysate
Lane 5 : NIH 3T3 cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 52 kDa
Exposure time: 3 seconds
Datasheets and documents
References (0)
ab186137 has not yet been referenced specifically in any publications.